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Applied and Environmental Microbiology, November 2009, p. 6886-6889, Vol. 75, No. 21
0099-2240/09/$08.00+0     doi:10.1128/AEM.01025-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

High-Level Resistance of Nosema ceranae, a Parasite of the Honeybee, to Temperature and Desiccation{triangledown}

S. Fenoy,1 C. Rueda,1 M. Higes,2 R. Martín-Hernández,2 and C. del Aguila1*

Laboratorio de Parasitología, Facultad de Farmacia, Universidad San Pablo CEU, Madrid, Spain,1 Centro Apícola Regional, Marchamalo, Guadalajara, Spain2

Received 5 May 2009/ Accepted 31 July 2009

Resistance of Nosema ceranae to different exposure conditions has been evaluated by using Sytox green and DAPI (4',6-diamidino-2-phenylindole) to test spore viability. High thermotolerance at 60 and 35°C and resistance to desiccation were observed. However, a significant decrease in viability after freezing and a rapid degeneration of spores maintained at 4°C were also detected.

* Corresponding author. Mailing address: Facultad de Farmacia, Urbanización Montepríncipe, 28668 Boadilla del Monte, Madrid, Spain. Phone: 34.91.372.47.96/84. Fax: 34.91.351.04.96. E-mail: cagupue{at}ceu.es

{triangledown} Published ahead of print on 4 September 2009.

Applied and Environmental Microbiology, November 2009, p. 6886-6889, Vol. 75, No. 21
0099-2240/09/$08.00+0     doi:10.1128/AEM.01025-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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