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Applied and Environmental Microbiology, November 2009, p. 6986-6991, Vol. 75, No. 22
0099-2240/09/$08.00+0     doi:10.1128/AEM.01605-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Thermostable Cyanuric Acid Hydrolase from Moorella thermoacetica ATCC 39073{triangledown}

Qingyan Li,1,{dagger} Jennifer L. Seffernick,2,3,4,{dagger} Michael J. Sadowsky,3,4,5 and Lawrence P. Wackett2,3,4*

Department of Microbiology and Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 30071, China,1 Department of Biochemistry, Molecular Biology, and Biophysics,2 Biotechnology Institute,3 Center for Microbial and Plant Genomics,4 Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota 551085

Received 7 July 2009/ Accepted 14 September 2009

Cyanuric acid, a metabolic intermediate in the degradation of many s-triazine compounds, is further metabolized by cyanuric acid hydrolase. Cyanuric acid also accumulates in swimming pools due to the breakdown of the sanitizing agents di- and trichloroisocyanuric acid. Structurally stable cyanuric acid hydrolases are being considered for usage in pool water remediation. In this study, cyanuric acid hydrolase from the thermophile Moorella thermoacetica ATCC 39073 was cloned, expressed in Escherichia coli, and purified to homogeneity. The recombinant enzyme was found to have a broader temperature range and greater stability, at both elevated and low temperatures, than previously described cyanuric acid hydrolases. The enzyme had a narrow substrate specificity, acting only on cyanuric acid and N-methylisocyanuric acid. The M. thermoacetica enzyme did not require metals or other discernible cofactors for activity. Cyanuric acid hydrolase from M. thermoacetica is the most promising enzyme to use for cyanuric acid remediation applications.

* Corresponding author. Mailing address: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St. Paul, MN 55108. Phone: (612) 625-3785. Fax: (612) 625-1700. E-mail: wacke003{at}umn.edu

{triangledown} Published ahead of print on 18 September 2009.

{dagger} Q.L. and J.L.S. contributed equally to this study.

Applied and Environmental Microbiology, November 2009, p. 6986-6991, Vol. 75, No. 22
0099-2240/09/$08.00+0     doi:10.1128/AEM.01605-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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