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Applied and Environmental Microbiology, November 2009, p. 7268-7270, Vol. 75, No. 22
0099-2240/09/$08.00+0     doi:10.1128/AEM.00135-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Statistical Assessment of Variability of Terminal Restriction Fragment Length Polymorphism Analysis Applied to Complex Microbial Communities {triangledown} ,{dagger}

Pierre Rossi,1* François Gillet,2 Emmanuelle Rohrbach,1 Nouhou Diaby,1 and Christof Holliger1

Ecole Polytechnique Fédérale de Lausanne (EPFL), ENAC ISTE Laboratory for Environmental Biotechnology, Lausanne, Switzerland,1 Ecole Polytechnique Fédérale de Lausanne, ENAC ISTE Laboratory of Ecosystems, Lausanne, Switzerland2

Received 20 January 2009/ Accepted 4 September 2009

The variability of terminal restriction fragment polymorphism analysis applied to complex microbial communities was assessed statistically. Recent technological improvements were implemented in the successive steps of the procedure, resulting in a standardized procedure which provided a high level of reproducibility.


* Corresponding author. Present address: EPFL ENAC ISTE-GR-CEL, Central Environmental Laboratory, CH C3 425, Station 6, CH-1015 Lausanne, Switzerland. E-mail: pierre.rossi{at}epfl.ch

{triangledown} Published ahead of print on 11 September 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.


Applied and Environmental Microbiology, November 2009, p. 7268-7270, Vol. 75, No. 22
0099-2240/09/$08.00+0     doi:10.1128/AEM.00135-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.