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Applied and Environmental Microbiology, December 2009, p. 7542-7547, Vol. 75, No. 23
0099-2240/09/$08.00+0     doi:10.1128/AEM.00814-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development and Application of Quantitative-PCR Tools for Subgroups of the Roseobacter Clade {triangledown}

Alison Buchan,1* Mary Hadden,1 and Marcelino T. Suzuki2,{dagger}

Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996,1 Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science, Solomons, Maryland 206882

Received 9 April 2009/ Accepted 25 September 2009

Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.

* Corresponding author. Mailing address: Department of Microbiology, M409 Walters Life Sciences, University of Tennessee, Knoxville, TN 37996. Phone: (865) 974-5234. Fax: (865) 974-4007. E-mail: abuchan{at}utk.edu

{triangledown} Published ahead of print on 2 October 2009.

{dagger} Present address: Observatoire Océanologique de Banyuls, Université Pierre et Marie Curie-Paris 6, UMR7621-INSUCNRS, BP44, F-66650 Banyuls-sur-Mer, France.

Applied and Environmental Microbiology, December 2009, p. 7542-7547, Vol. 75, No. 23
0099-2240/09/$08.00+0     doi:10.1128/AEM.00814-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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