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Applied and Environmental Microbiology, February 2009, p. 589-595, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.00973-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Production of Glucaric Acid from a Synthetic Pathway in Recombinant Escherichia coli{triangledown} ,{dagger}

Tae Seok Moon, Sang-Hwal Yoon, Amanda M. Lanza, Joseph D. Roy-Mayhew, and Kristala L. Jones Prather*

Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts

Received 29 April 2008/ Accepted 30 November 2008

A synthetic pathway has been constructed for the production of glucuronic and glucaric acids from glucose in Escherichia coli. Coexpression of the genes encoding myo-inositol-1-phosphate synthase (Ino1) from Saccharomyces cerevisiae and myo-inositol oxygenase (MIOX) from mice led to production of glucuronic acid through the intermediate myo-inositol. Glucuronic acid concentrations up to 0.3 g/liter were measured in the culture broth. The activity of MIOX was rate limiting, resulting in the accumulation of both myo-inositol and glucuronic acid as final products, in approximately equal concentrations. Inclusion of a third enzyme, uronate dehydrogenase (Udh) from Pseudomonas syringae, facilitated the conversion of glucuronic acid to glucaric acid. The activity of this recombinant enzyme was more than 2 orders of magnitude higher than that of Ino1 and MIOX and increased overall flux through the pathway such that glucaric acid concentrations in excess of 1 g/liter were observed. This represents a novel microbial system for the biological production of glucaric acid, a "top value-added chemical" from biomass.

* Corresponding author. Mailing address: 77 Massachusetts Avenue, Room 66-458, Cambridge, MA 02139. Phone: (617) 253-1950. Fax: (617) 258-5042. E-mail: kljp{at}mit.edu

{triangledown} Published ahead of print on 5 December 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, February 2009, p. 589-595, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.00973-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yoon, S.-H., Moon, T. S., Iranpour, P., Lanza, A. M., Prather, K. J. (2009). Cloning and Characterization of Uronate Dehydrogenases from Two Pseudomonads and Agrobacterium tumefaciens Strain C58. J. Bacteriol. 191: 1565-1573 [Abstract] [Full Text]  


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