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Applied and Environmental Microbiology, February 2009, p. 869-871, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01728-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Enhanced Secretion of Biologically Active Murine Interleukin-12 by Lactococcus lactis{triangledown}

Antonio Fernandez,1* Nikki Horn,1* Udo Wegmann,1 Claudio Nicoletti,2 Michael J. Gasson,1 and Arjan Narbad1

Commensals and Microflora,1 Gastrointestinal Tract Biology and Health, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom2

Received 28 July 2008/ Accepted 25 November 2008

The novel signal peptide SLPmod was used for the secretion of murine interleukin-12 (mIL-12) by Lactococcus lactis. A >4-fold increase in secretion was observed when SLPmod was used instead of the Usp45-derived secretion signal. Oral delivery of this cytokine using the autoinducible host L. lactis FI5876 utilizing SLPmod resulted in a significant increase in mIL-12 plasma levels in mice.

* Corresponding author. Mailing address: Commensals and Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. Phone: 441603-255000. Fax: 441603-255288. E-mail for A. Fernandez: antonio.fernandezdumont{at}efsa.europa.eu. E-mail for N. Horn: nikki.horn{at}bbsrc.ac.uk

{triangledown} Published ahead of print on 5 December 2008.

Applied and Environmental Microbiology, February 2009, p. 869-871, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01728-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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