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Applied and Environmental Microbiology, February 2009, p. 875-877, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01682-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Analysis of Dibenzothiophene Desulfurization in a Recombinant Pseudomonas putida Strain{triangledown}

Javier Calzada,1 María T. Zamarro,2 Almudena Alcón,1 Victoria E. Santos,1* Eduardo Díaz,2 José L. García,2 and Felix Garcia-Ochoa1

Departamento de Ingeniería Química, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, 28040 Madrid,1 Departamento de Microbiología Molecular, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, 28040 Madrid, Spain2

Received 21 July 2008/ Accepted 17 November 2008

Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early exponential growth phases was designed to significantly increase biodesulfurization.

* Corresponding author. Mailing address: Departamento de Ingeniería Química, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Ciudad Universitaria s/n, 28040 Madrid, Spain. Phone: 34-913944179. Fax: 34-913944171. E-mail: vesantos{at}quim.ucm.es

{triangledown} Published ahead of print on 1 December 2008.

Applied and Environmental Microbiology, February 2009, p. 875-877, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01682-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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