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Applied and Environmental Microbiology, February 2009, p. 882-884, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01924-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Innovative Application of Mass Spectrometry for the Characterization of Staphylococcal Enterotoxins Involved in Food Poisoning Outbreaks{triangledown}

Jacques-Antoine Hennekinne,1* Virginie Brun,2 Marie-Laure De Buyser,1 Alain Dupuis,2 Annick Ostyn,1 and Sylviane Dragacci1

AFSSA-LERQAP (French Agency for Food Safety, Laboratory for Study and Research on Quality of Food and on Food Processes), European Union Community Reference Laboratory for Coagulase Positive Staphylococci Including Staphylococcus aureus, 23 avenue du Général De Gaulle, 94706 Maisons-Alfort cedex, France,1 CEA, DSV, iRTSV, Laboratoire d'Etude de la Dynamique des Protéomes, F-38054 Grenoble, France, INSERM, U880, F-38054 Grenoble, France, and Université Joseph Fourier, F-38054 Grenoble, France2

Received 19 August 2008/ Accepted 1 December 2008

Staphylococcal poisoning is a common food-borne disease for which immunoassays to detect enterotoxins were developed, but these assays often lead to false diagnoses due to interferences or lack of specificity. Absolute quantitative mass spectrometry was for the first time successfully applied to an investigation of a staphylococcal outbreak due to coconut pearls.

* Corresponding author. Mailing address: AFSSA-LERQAP, TOP BAC, 23 avenue du Général De Gaulle, 94706 Maisons-Alfort cedex, France. Phone: 33 1 49 77 46 83. Fax: 33 1 49 77 46 66. E-mail: ja.hennekinne{at}afssa.fr

{triangledown} Published ahead of print on 12 December 2008.

Applied and Environmental Microbiology, February 2009, p. 882-884, Vol. 75, No. 3
0099-2240/09/$08.00+0     doi:10.1128/AEM.01924-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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