This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Toyota-Hanatani, Y.
Right arrow Articles by Baba, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Toyota-Hanatani, Y.
Right arrow Articles by Baba, E.
Agricola
Right arrow Articles by Toyota-Hanatani, Y.
Right arrow Articles by Baba, E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2009, p. 1005-1010, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.01689-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Public Health Assessment of Salmonella enterica Serovar Enteritidis Inactivated-Vaccine Treatment in Layer Flocks{triangledown}

Yukiko Toyota-Hanatani,1 Tomoya Ekawa,1 Hiroaki Ohta,1* Shizunobu Igimi,2 Yukiko Hara-Kudo,2 Kazumi Sasai,3 and Eiichiroh Baba3

CAF Laboratories, 1257-1 Michinoue-Kannabe, Fukuyama, Hiroshima 720-2104, Japan,1 National Institute of Health Science, Ministry of Health Labor and Welfare, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan,2 Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, 1-1 Gakuencho, Sakai, Osaka 599-8531, Japan3

Received 22 July 2008/ Accepted 23 November 2008

Although there have been several reports on the efficacy assessment of a Salmonella enterica serovar Enteritidis vaccine against intestinal and parenchymatous organ diseases of laying hens, no public health risk characterization of its long-term effect on eggs has been reported. In this study, we attempted to assess the public health effect of an inactivated S. enterica serovar Enteritidis vaccine against serovar Enteritidis contamination of chicken eggs. We analyzed serovar Enteritidis isolation test results from four windowless farms in which inactivated-vaccine administration was initiated based on the sanitary monitoring program of a farm. When flocks with and without S. enterica serovar Enteritidis vaccine treatments were mixed, the application of an inactivated serovar Enteritidis vaccine decreased the most probable number (MPN) of bacteria by at least 100-fold in broken (liquid) egg samples positive for serovar Enteritidis, although a statistical difference between those MPNs could not be obtained. The isolation frequency after the vaccine application was less than 1/10 (P < 0.01). No S. enterica serovar Enteritidis bacteria were isolated approximately 1 year after all of the chickens had received the inactivated serovar Enteritidis vaccine. It was suggested that an adequate administration of an inactivated serovar Enteritidis vaccine reduced the contamination risk of eggs (the number of isolated serovar Enteritidis cells and detection frequency) compared to the contamination risk of eggs laid by nonvaccinated hens.

* Corresponding author. Mailing address: CAF Laboratories, 1257-1 Michinoue-Kannabe, Fukuyama, Hiroshima 720-2104, Japan. Phone: (81) 84-963-4228. Fax: (81) 84-963-4265. E-mail: caf-laboh{at}pop21.odn.ne.jp

{triangledown} Published ahead of print on 1 December 2008.

Applied and Environmental Microbiology, February 2009, p. 1005-1010, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.01689-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»