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Applied and Environmental Microbiology, February 2009, p. 1135-1143, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.02391-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of Two Novel {alpha}-Glucosidases from Bifidobacterium breve UCC2003{triangledown} ,{dagger}

Karina Pokusaeva,1,2 Mary O'Connell-Motherway,1,2 Aldert Zomer,1 Gerald F. Fitzgerald,1,2,3 and Douwe van Sinderen1,2*

Alimentary Pharmabiotic Centre,1 Department of Microbiology,2 Food and Nutritional Sciences, University College Cork, Western Road, Cork, Ireland3

Received 17 October 2008/ Accepted 16 December 2008

Two {alpha}-glucosidase-encoding genes (agl1 and agl2) from Bifidobacterium breve UCC2003 were identified and characterized. Based on their similarity to characterized carbohydrate hydrolases, the Agl1 and Agl2 enzymes are both assigned to a subgroup of the glycosyl hydrolase family 13, the {alpha}-1,6-glucosidases (EC 3.2.1.10). Recombinant Agl1 and Agl2 into which a His12 sequence was incorporated (Agl1His and Agl2His, respectively) exhibited hydrolytic activity towards panose, isomaltose, isomaltotriose, and four sucrose isomers—palatinose, trehalulose, turanose, and maltulose—while also degrading trehalose and, to a lesser extent, nigerose. The preferred substrates for both enzymes were panose, isomaltose, and trehalulose. Furthermore, the pH and temperature optima for both enzymes were determined, showing that Agl1His exhibits higher thermo and pH optima than Agl2His. The two purified {alpha}-1,6-glucosidases were also shown to have transglycosylation activity, synthesizing oligosaccharides from palatinose, trehalulose, trehalose, panose, and isomaltotriose.

* Corresponding author. Mailing address: Room 4.05, Department of Microbiology, University College Cork, Western Road, Cork, Ireland. Phone: 353 21 490 1365. Fax: 353 21 490 3101. E-mail: d.vansinderen{at}ucc.ie

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, February 2009, p. 1135-1143, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.02391-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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