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Applied and Environmental Microbiology, February 2009, p. 1192-1196, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.02054-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Class 1 Integron Resistance Gene Cassettes among Salmonella Strains Isolated from Imported Seafood {triangledown}

Ashraf A. Khan,1* Elizabeth Ponce,2 M. S. Nawaz,1 Chorng-Ming Cheng,3 Junaid A. Khan,4 and Christine S. West1

Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, Arkansas 72079,1 Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Departamento de Biotecnología Marina, Km. 107 carretera Tijuana-Ensenada, Ensenada, B.C. 22860, Mexico,2 Pacific Regional Laboratory-Southwest, U.S. Food and Drug Administration, Irvine, California,3 Department of Cardiology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 722054

Received 4 September 2008/ Accepted 1 December 2008

A total of 210 Salmonella isolates, representing 64 different serovars, were isolated from imported seafood samples, and 55/210 isolates were found to be resistant to at least one antibiotic. Class 1 integrons from three multidrug-resistant Salmonella enterica strains (Salmonella enterica serovars Newport [strain 62], Typhimurium var. Copenhagen [strain 629], and Lansing [strain 803], originating from Hong Kong, the Philippines, and Taiwan, respectively) were characterized. Southern hybridization of plasmids isolated from these strains, using a class 1 integron probe, showed that trimethoprim-sulfamethoxazole and streptomycin resistance genes were located on a megaplasmid in strain 629. Our study indicates that imported seafood could be a reservoir for Salmonella isolates resistant to multiple antibiotics.

* Corresponding author. Mailing address: Division of Microbiology, U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR 72079. Phone: (870) 543-7601. Fax: (870) 543-7307. E-mail: Ashraf.khan{at}fda.hhs.gov

{triangledown} Published ahead of print on 12 December 2008.

Applied and Environmental Microbiology, February 2009, p. 1192-1196, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.02054-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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