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Applied and Environmental Microbiology, February 2009, p. 1204-1207, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.00908-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Bile Affects the Synthesis of Exopolysaccharides by Bifidobacterium animalis{triangledown}

Patricia Ruas-Madiedo,1* Miguel Gueimonde,1 Fabrizio Arigoni,2 Clara G. de los Reyes-Gavilán,1 and Abelardo Margolles1

Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain,1 Nestlé Research Centre, CH-1000 Lausanne 26, Switzerland2

Received 22 April 2008/ Accepted 10 December 2008

By using cryo-scanning electron microscopy and quantification with lectin-conjugated probes, we have detected the production of exopolysaccharides (EPS) in Bifidobacterium animalis subsp. lactis in the presence of bile. In addition, the expression of gtf01207, which codifies a putative priming glycosyltransferase involved in EPS synthesis, was induced by bile.


* Corresponding author. Mailing address: Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Carretera de Infiesto s/n 33300 Villaviciosa, Asturias, Spain. Phone: 34 985892131. Fax: 34 985892233. E-mail: ruas-madiedo{at}ipla.csic.es

{triangledown} Published ahead of print on 16 December 2008.


Applied and Environmental Microbiology, February 2009, p. 1204-1207, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.00908-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.