This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chaillou, S.
Right arrow Articles by Zagorec, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chaillou, S.
Right arrow Articles by Zagorec, M.
Agricola
Right arrow Articles by Chaillou, S.
Right arrow Articles by Zagorec, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2009, p. 970-980, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.01721-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Intraspecies Genomic Diversity and Natural Population Structure of the Meat-Borne Lactic Acid Bacterium Lactobacillus sakei{triangledown} ,{dagger}

Stéphane Chaillou,1* Marie Daty,1 Fabienne Baraige,1 Anne-Marie Dudez,1,{ddagger} Patricia Anglade,1 Rhys Jones,1,2 Carl-Alfred Alpert,1,§ Marie-Christine Champomier-Vergès,1 and Monique Zagorec1

UR309, Unité Flore Lactique & Environnement Carné (FLEC), Institut National de la Recherche Agronomique (INRA), Domaine de Vilvert, 78350 Jouy-en-Josas, France,1 Food, Metabolism and Microbiology, AgResearch Ltd., Private Bag 3123, Hamilton 3240, New Zealand2

Received 25 July 2008/ Accepted 16 December 2008

Lactobacillus sakei is a food-borne bacterium naturally found in meat and fish products. A study was performed to examine the intraspecies diversity among 73 isolates sourced from laboratory collections in several different countries. Pulsed-field gel electrophoresis analysis demonstrated a 25% variation in genome size between isolates, ranging from 1,815 kb to 2,310 kb. The relatedness between isolates was then determined using a PCR-based method that detects the possession of 60 chromosomal genes belonging to the flexible gene pool. Ten different strain clusters were identified that had noticeable differences in their average genome size reflecting the natural population structure. The results show that many different genotypes may be isolated from similar types of meat products, suggesting a complex ecological habitat in which intraspecies diversity may be required for successful adaptation. Finally, proteomic analysis revealed a slight difference between the migration patterns of highly abundant GapA isoforms of the two prevailing L. sakei subspecies (sakei and carnosus). This analysis was used to affiliate the genotypic clusters with the corresponding subspecies. These findings reveal for the first time the extent of intraspecies genomic diversity in L. sakei. Consequently, identification of molecular subtypes may in the future prove valuable for a better understanding of microbial ecosystems in food products.

* Corresponding author. Mailing address: Unité Flore Lactique & Environnement Carné (FLEC), Institut National de la Recherche Agronomique (INRA), Domaine de Vilvert, 78350 Jouy-en-Josas, France. Phone: 33 134 65 21 06. Fax: 33 134 65 21 05. E-mail: Stephane.Chaillou{at}jouy.inra.fr

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

{ddagger} Present address: UMR INRA/CNRS: Interactions Plantes-Microorganismes INRA Chemin de Borde-Rouge, Auzeville BP 52627, 31326 Castanet-Tolosan Cedex, France.

§ Present address: Deutsches Institut für Ernährungsforschung, Abteilung Gastrointestinale Mikrobiologie, Arthur-Seheunert Allee 114-115, 14558 Bergholtz-Rihbrücke, Germany.

Applied and Environmental Microbiology, February 2009, p. 970-980, Vol. 75, No. 4
0099-2240/09/$08.00+0     doi:10.1128/AEM.01721-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, Z.-Y., Liu, C., Zhu, Y.-Z., Zhong, Y., Zhu, Y.-Q., Zheng, H.-J., Zhao, G.-P., Wang, S.-Y., Guo, X.-K. (2009). Complete Genome Sequence of Lactobacillus plantarum JDM1. J. Bacteriol. 191: 5020-5021 [Abstract] [Full Text]  
  • Chiaramonte, F., Blugeon, S., Chaillou, S., Langella, P., Zagorec, M. (2009). Behavior of the Meat-Borne Bacterium Lactobacillus sakei during Its Transit through the Gastrointestinal Tracts of Axenic and Conventional Mice. Appl. Environ. Microbiol. 75: 4498-4505 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»