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Applied and Environmental Microbiology, March 2009, p. 1236-1242, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01598-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Growth-Regulated Expression of a Bacteriocin, Produced by the Sweet Potato Pathogen Streptomyces ipomoeae, That Exhibits Interstrain Inhibition{triangledown}

Jing Wang,1 Kevin L. Schully,1,{dagger} and Gregg S. Pettis1,2*

Department of Biological Sciences, Louisiana State University,1 Louisiana State University Agricultural Center, Louisiana Agricultural Experiment Station, Baton Rouge, Louisiana 708032

Received 11 July 2008/ Accepted 19 December 2008

Certain strains of the bacterial sweet potato pathogen Streptomyces ipomoeae produce the bacteriocin ipomicin, which inhibits other sensitive strains of the same species. Within the signal-sequence-encoding portion of the ipomicin structural gene ipoA exists a single rare TTA codon, which is recognized in Streptomyces bacteria by the temporally accumulating bldA leucyl tRNA. In this study, ipomicin was shown to stably accumulate in culture supernatants of S. ipomoeae in a growth-regulated manner that did not coincide with the pattern of ipoA expression. Similar growth-regulated production of ipomicin in Streptomyces coelicolor containing the cloned ipoA gene was found to be directly dependent on translation of the ipoA TTA codon by the bldA leucyl tRNA. The results here suggest that bldA-dependent translation of the S. ipomoeae ipoA gene leads to growth-regulated production of the ipomicin precursor, which upon processing to the mature form and secretion stably accumulates in the extracellular environment. To our knowledge, this is the first example of bldA regulation of a bacteriocin in the streptomycetes.

* Corresponding author. Mailing address: Department of Biological Sciences, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA 70803. Phone: (225) 578-2798. Fax: (225) 578-2597. E-mail: gpettis{at}lsu.edu

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Present address: Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21704.

Applied and Environmental Microbiology, March 2009, p. 1236-1242, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01598-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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