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Applied and Environmental Microbiology, March 2009, p. 1363-1372, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01512-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Fermentation of Barley by Using Saccharomyces cerevisiae: Examination of Barley as a Feedstock for Bioethanol Production and Value-Added Products

Amera Gibreel, James R. Sandercock, Jingui Lan, Laksiri A. Goonewardene, Ruurd T. Zijlstra, Jonathan M. Curtis, and David C. Bressler^*

Department of Agriculture, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2H7, Canada

Received 3 July 2008/ Accepted 1 December 2008

The objective of this study was to examine the ethanol yield potential of three barley varieties (Xena, Bold, and Fibar) in comparison to two benchmarks, corn and wheat. Very high gravity (VHG; 30% solids) fermentations using both conventional and Stargen 001 enzymes for starch hydrolysis were carried out as simultaneous saccharification and fermentation. The grains and their corresponding dried distiller's grain with solubles (DDGS) were also analyzed for nutritional and value-added characteristics. A VHG traditional fermentation approach utilizing jet-cooking fermentation revealed that both dehulled Bold and Xena barley produced ethanol concentrations higher than that produced by wheat (12.3, 12.2, and 11.9%, respectively) but lower than that produced by corn (13.8%). VHG-modified Stargen-based fermentation of dehulled Bold barley demonstrated comparable performance (14.3% ethanol) relative to that of corn (14.5%) and wheat (13.3%). Several important components were found to survive fermentation and were concentrated in DDGS. The highest yield of phenolics was detected in the DDGS (modified Stargen 001, 20% solids) of Xena (14.6 mg of gallic acid/g) and Bold (15.0 mg of gallic acid/g) when the hull was not removed before fermentation. The highest concentration of sterols in DDGS from barley was found in Xena (3.9 mg/g) when the hull was included. The DDGS recovered from corn had the highest concentration of fatty acids (72.6 and 77.5 mg/g). The DDGS recovered from VHG jet-cooking fermentations of Fibar, dehulled Bold, and corn demonstrated similar levels of tocopherols and tocotrienols. Corn DDGS was highest in crude fat but was lowest in crude protein and in vitro energy digestibility. Wheat DDGS was highest in crude protein content, similar to previous studies. The barley DDGS was the highest in in vitro energy digestibility.

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* Corresponding author. Mailing address: Department of Agriculture, Food and Nutritional Science, University of Alberta, 410 Agriculture Forestry Bldg., Edmonton, Alberta T6G 2H7, Canada. Phone: (780) 492-4986. Fax: (780) 492-4265. E-mail: david.bressler{at}ualberta.ca

Published ahead of print on 29 December 2008.

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Applied and Environmental Microbiology, March 2009, p. 1363-1372, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.01512-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.