This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zbell, A. L.
Right arrow Articles by Maier, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zbell, A. L.
Right arrow Articles by Maier, R. J.
Agricola
Right arrow Articles by Zbell, A. L.
Right arrow Articles by Maier, R. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2009, p. 1456-1459, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.02064-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of the Hya Hydrogenase in Recycling of Anaerobically Produced H2 in Salmonella enterica Serovar Typhimurium{triangledown}

Andrea L. Zbell and Robert J. Maier*

Department of Microbiology, University of Georgia, Athens, Georgia

Received 5 September 2008/ Accepted 21 December 2008

Double and triple uptake-type hydrogenase mutants were used to determine which hydrogenase recycles fermentatively produced hydrogen. The {Delta}hyb {Delta}hya and {Delta}hyd {Delta}hya double mutants evolved H2 at rates similar to that of the triple mutant strain, so Hya alone oxidizes the bulk of H2 produced during fermentation. When only Hya was present, no hydrogen production was observed in nutrient-limited medium. H2 uptake assays showed that Hya can oxidize both exogenously added H2 and formate hydrogen lyase-evolved H2 anaerobically. Even after anaerobic growth, all three uptake-type hydrogenases could function in the presence of oxygen, including using O2 as a terminal acceptor.

* Corresponding author. Mailing address: Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602. Phone: (706) 542-2323. Fax: (706) 542-2674. E-mail: rmaier{at}uga.edu

{triangledown} Published ahead of print on 29 December 2008.

Applied and Environmental Microbiology, March 2009, p. 1456-1459, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.02064-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»