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Applied and Environmental Microbiology, March 2009, p. 1460-1464, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.02096-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Targeted Engineering of the Antibacterial Peptide Apidaecin, Based on an In Vivo Monitoring Assay System{triangledown}

Seiichi Taguchi,1* Kensuke Mita,1 Kenta Ichinohe,1 and Shigeki Hashimoto2

Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo-shi, Hokkaido 060-8628, Japan,1 Faculty of Industrial Science and Technology, Tokyo University of Science, Oshamanbe, Hokkaido 049-3514, Japan2

Received 10 September 2008/ Accepted 22 December 2008

Seven mutant forms of the antibacterial peptide apidaecin with increased activity were created by combinatorial mutagenesis targeted to the three N-terminal amino acid residues that had previously been identified as a nonessential region. An in vitro MIC assay revealed that the amino acid substitutions in the functionally variable region were effective in improving differential activity toward the four gram-negative bacteria tested, while a gram-positive bacterium was unaffected.

* Corresponding author. Mailing address: Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo-shi, Hokkaido 060-8628, Japan. Phone and fax: 81-11-706-6610. E-mail: staguchi{at}eng.hokudai.ac.jp

{triangledown} Published ahead of print on 29 December 2008.

Applied and Environmental Microbiology, March 2009, p. 1460-1464, Vol. 75, No. 5
0099-2240/09/$08.00+0     doi:10.1128/AEM.02096-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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