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Next Article 

Applied and Environmental Microbiology, March 2009, p. 1471-1477, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of proP and proU in Betaine Uptake by Yersinia enterocolitica under Cold and Osmotic Stress Conditions{triangledown} ,{dagger}

Thirunavukkarasu Annamalai1 and Kumar Venkitanarayanan2*

Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, New York 10595,1 Department of Animal Science, Unit 4040, University of Connecticut, Storrs, Connecticut 062692

Received 17 July 2008/ Accepted 21 December 2008

Yersinia enterocolitica is a food-borne pathogen with the ability to grow at cold temperatures and tolerate high osmolarity. The bacterium tolerates osmotic stress by intracellular accumulation of osmolytes, such as betaine. The proP gene and proU operon of Y. enterocolitica were sequenced, and single (ProP– ProU+ and ProP+ ProU–) and double (ProP– ProU–) mutants were generated. Upon exposure to osmotic or chill stress, the single and double mutants demonstrated a reduction in betaine uptake compared to that in the wild type, suggesting that proP and proU play a role in betaine uptake during osmotic and chill stress responses of Y. enterocolitica.

* Corresponding author. Mailing address: Department of Animal Science, Unit 4040, 3636 Horsebarn Hill Road Extension, Storrs, CT 06269. Phone: (860) 486-0947. Fax: (860) 486-4375. E-mail: kumar.venkitanarayanan{at}uconn.edu

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, March 2009, p. 1471-1477, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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