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Applied and Environmental Microbiology, March 2009, p. 1534-1545, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02216-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract{triangledown}

Francesca Turroni,1 Elena Foroni,1 Paola Pizzetti,1 Vanessa Giubellini,1 Angela Ribbera,1 Paolo Merusi,2 Patrizio Cagnasso,2 Barbara Bizzarri,3 Gian Luigi de'Angelis,3 Fergus Shanahan,4 Douwe van Sinderen,4 and Marco Ventura1*

Department of Genetics, Biology of Microorganisms, Anthropology and Evolution, University of Parma, Parma, Italy,1 Parmalat Research Center, Parmalat SpA, Parma, Italy,2 Pediatric Gastroenterology Unit, University of Parma, Parma, Italy,3 Alimentary Pharmabiotic Centre and Department of Microbiology, Bioscience Institute, National University of Ireland, Western Road, Cork, Ireland4

Received 25 September 2008/ Accepted 12 January 2009

Although the health-promoting roles of bifidobacteria are widely accepted, the diversity of bifidobacteria among the human intestinal microbiota is still poorly understood. We performed a census of bifidobacterial populations from human intestinal mucosal and fecal samples by plating them on selective medium, coupled with molecular analysis of selected rRNA gene sequences (16S rRNA gene and internally transcribed spacer [ITS] 16S-23S spacer sequences) of isolated colonies. A total of 900 isolates were collected, of which 704 were shown to belong to bifidobacteria. Analyses showed that the culturable bifidobacterial population from intestinal and fecal samples include six main phylogenetic taxa, i.e., Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium adolescentis, Bifidobacterium pseudolongum, Bifidobacterium breve, and Bifidobacterium bifidum, and two species mostly detected in fecal samples, i.e., Bifidobacterium dentium and Bifidobacterium animalis subp. lactis. Analysis of bifidobacterial distribution based on age of the subject revealed that certain identified bifidobacterial species were exclusively present in the adult human gut microbiota whereas others were found to be widely distributed. We encountered significant intersubject variability and composition differences between fecal and mucosa-adherent bifidobacterial communities. In contrast, a modest diversification of bifidobacterial populations was noticed between different intestinal regions within the same individual (intrasubject variability). Notably, a small number of bifidobacterial isolates were shown to display a wide ecological distribution, thus suggesting that they possess a broad colonization capacity.

* Corresponding author. Mailing address: Department of Genetics, Anthropology and Evolution, University of Parma, Parco Area delle Scienze 11a, 43100 Parma, Italy. Phone: 39-521-905666. Fax: 39-521-905476. E-mail: marco.ventura{at}unipr.it

{triangledown} Published ahead of print on 23 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1534-1545, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02216-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Barrangou, R., Briczinski, E. P., Traeger, L. L., Loquasto, J. R., Richards, M., Horvath, P., Coute-Monvoisin, A.-C., Leyer, G., Rendulic, S., Steele, J. L., Broadbent, J. R., Oberg, T., Dudley, E. G., Schuster, S., Romero, D. A., Roberts, R. F. (2009). Comparison of the Complete Genome Sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04. J. Bacteriol. 191: 4144-4151 [Abstract] [Full Text]  


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