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Applied and Environmental Microbiology, March 2009, p. 1552-1558, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02299-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification and Characterization of Lactocyclicin Q, a Novel Cyclic Bacteriocin Produced by Lactococcus sp. Strain QU 12{triangledown}

Naruhiko Sawa,1 Takeshi Zendo,1 Junko Kiyofuji,1 Koji Fujita,1 Kohei Himeno,1 Jiro Nakayama,1 and Kenji Sonomoto1,2*

Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School,1 Laboratory of Functional Food Design, Department of Functional Metabolic Design, Bio-Architecture Center, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan2

Received 7 October 2008/ Accepted 30 December 2008

Lactococcus sp. strain QU 12, which was isolated from cheese, produced a novel cyclic bacteriocin termed lactocyclicin Q. By using cation-exchange chromatography, hydrophobic interaction chromatography, and reverse-phase high-performance liquid chromatography, lactocyclicin Q was purified from culture supernatant, and its molecular mass was determined to be 6,062.8 Da by mass spectrometry. Lactocyclicin Q has been characterized by its unique antimicrobial spectrum, high level of protease resistance, and heat stability compared to other reported bacteriocins of lactic acid bacteria. The amino acid sequence of lactocyclicin Q was determined chemically, and this compound is composed of 61 amino acid residues that have a cyclic structure with linkage between the N and C termini by a peptide bond. It showed no homology to any other antimicrobial peptide, including cyclic bacteriocins. On the basis of the amino acid sequences obtained, the sequence of the gene encoding the prepeptide lactocyclicin Q was obtained. This is the first report of a cyclic bacteriocin purified from a strain belonging to the genus Lactococcus.

* Corresponding author. Mailing address: Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Phone: (81) 92 642 3019. Fax: (81) 92 642 3019. E-mail: sonomoto{at}agr.kyushu-u.ac.jp

{triangledown} Published ahead of print on 9 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1552-1558, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02299-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Martin-Visscher, L. A., Gong, X., Duszyk, M., Vederas, J. C. (2009). The Three-dimensional Structure of Carnocyclin A Reveals That Many Circular Bacteriocins Share a Common Structural Motif. J. Biol. Chem. 284: 28674-28681 [Abstract] [Full Text]  


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