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Applied and Environmental Microbiology, March 2009, p. 1688-1696, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01210-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Molecular Diversity of a North Carolina Wastewater Treatment Plant as Revealed by Pyrosequencing{triangledown} ,{dagger}

Nina Sanapareddy,1 Timothy J. Hamp,1 Luis C. Gonzalez,1 Helene A. Hilger,2 Anthony A. Fodor,1* and Sandra M. Clinton3

Bioinformatics Research Center,1 Department of Civil and Environmental Engineering,2 Department of Biology, University of North Carolina at Charlotte, 9201 University City Blvd., Charlotte, North Carolina 282233

Received 30 May 2008/ Accepted 16 December 2008

We report the results of pyrosequencing of DNA collected from the activated sludge basin of a wastewater treatment plant in Charlotte, NC. Using the 454-FLX technology, we generated 378,601 sequences with an average read length of 250.4 bp. Running the 454 assembly algorithm over our sequences yielded very poor assembly, with only 0.3% of our sequences participating in assembly of significant contigs. Of the 117 contigs greater than 500 bp long that were assembled, the most common annotations were to transposases and hypothetical proteins. Comparing our sequences to known microbial genomes showed nonspecific recruitment, indicating that previously described taxa are only distantly related to the most abundant microbes in this treatment plant. A comparison of proteins generated by translating our sequence set to translations of other sequenced microbiomes shows a distinct metabolic profile for activated sludge with high counts for genes involved in metabolism of aromatic compounds and low counts for genes involved in photosynthesis. Taken together, these data document the substantial levels of microbial diversity within activated sludge and further establish the great utility of pyrosequencing for investigating diversity in complex ecosystems.

* Corresponding author. Mailing address: Bioinformatics Research Center, Cameron 212, UNC Charlotte, 9201 University City Blvd., Charlotte, NC 28223. Phone: (704) 687-8214. Fax: (704) 687-6610. E-mail: anthony.fodor{at}gmail.com

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, March 2009, p. 1688-1696, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.01210-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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