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Applied and Environmental Microbiology, March 2009, p. 1734-1739, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02000-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Image Analysis Software Based on Color Segmentation for Characterization of Viability and Physiological Activity of Biofilms{triangledown}

Luis E. Chávez de Paz*

Department of Oral Biology, Faculty of Odontology, Malmö University, Malmö 20506, Sweden

Received 28 August 2008/ Accepted 4 January 2009

The novel image analysis software package bioImage_L was tested to calculate biofilm structural parameters in oral biofilms stained with dual-channel fluorescent markers. By identifying color tonalities in situ, the software independently processed the color subpopulations and characterized the viability and metabolic activity of biofilms.

* Mailing address: Department of Oral Biology, Faculty of Odontology, Malmö University, Malmö SE-20506, Sweden. Phone: 46 40 6658659. Fax: 46 40 929359. E-mail: luis.chavez.de.paz{at}od.mah.se

{triangledown} Published ahead of print on 9 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1734-1739, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02000-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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