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Applied and Environmental Microbiology, March 2009, p. 1750-1753, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02279-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Diffusion of Macromolecules in Model Oral Biofilms{triangledown}

Shoji Takenaka,1,2 Betsey Pitts,2 Harsh M. Trivedi,3 and Philip S. Stewart2*

Division of Cariology, Niigata University, Niigata, Japan,1 Center for Biofilm Engineering, Montana State University—Bozeman, Bozeman, Montana 59717-3980,2 Colgate-Palmolive Technology Center, Piscataway, New Jersey3

Received 3 October 2008/ Accepted 8 January 2009

The diffusive penetration of fluorescently tagged macromolecular solutes into model oral biofilms was visualized by time-lapse microscopy. All of the solutes tested, including dextrans, proteases, green fluorescent protein, and immunoglobulin G, accessed the interior of cell clusters 100 to 200 µm in diameter within 3 min or less.

* Corresponding author. Mailing address: Center for Biofilm Engineering, Montana State University—Bozeman, Bozeman, MT 59717-3980. Phone: (406) 994-4770. Fax: (406) 994-6098. E-mail: phil_s{at}biofilm.montana.edu

{triangledown} Published ahead of print on 23 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1750-1753, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02279-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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