This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fan, Z.
Right arrow Articles by Yuan, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fan, Z.
Right arrow Articles by Yuan, L.
Agricola
Right arrow Articles by Fan, Z.
Right arrow Articles by Yuan, L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2009, p. 1754-1757, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02181-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Multimeric Hemicellulases Facilitate Biomass Conversion{triangledown} ,{dagger}

Zhanmin Fan,1,2 Kurt Wagschal,3 Wei Chen,4 Michael D. Montross,4 Charles C. Lee,3 and Ling Yuan1,2*

Department of Plant and Soil Sciences,1 Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, Kentucky 40546,2 USDA Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, California 94710,3 Department of Biosystems and Agricultural Engineering, University of Kentucky, Lexington, Kentucky 405464

Received 20 September 2008/ Accepted 10 January 2009

Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover.

* Corresponding author. Mailing address: Department of Plant and Soil Sciences, Kentucky Tobacco Research and Development Center, University of Kentucky, Cooper and University Drives, Lexington, KY 40546. Phone: (859) 257-4086. Fax: (589) 323-1077. E-mail: lyuan3{at}uky.edu

{triangledown} Published ahead of print on 16 January 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, March 2009, p. 1754-1757, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02181-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»