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Applied and Environmental Microbiology, March 2009, p. 1764-1768, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02273-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Phage-Mediated Shiga Toxin 2 Gene Transfer in Food and Water{triangledown}

Lejla Imamovic,1 Juan Jofre,1 Herbert Schmidt,2 Ruth Serra-Moreno,1 and Maite Muniesa1*

Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain,1 Institute of Food Science and Biotechnology, Department of Food Microbiology, University of Hohenheim, Garbenstrasse 28, 70599 Stuttgart, Germany2

Received 3 October 2008/ Accepted 12 January 2009

Shiga toxin (stx) transduction in various food matrices has been evaluated with lysogens of Stx phages. stx transduction events were observed for many phages under appropriate conditions. Transduction did not occur at low pH and low temperatures. A total of 103 to 104 CFU ml–1 was the minimal amount of donor and recipient strains necessary to generate transductants.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Biology, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain. Phone: 34934039386. Fax: 34934039047. E-mail: mmuniesa{at}ub.edu

{triangledown} Published ahead of print on 23 January 2009.

Applied and Environmental Microbiology, March 2009, p. 1764-1768, Vol. 75, No. 6
0099-2240/09/$08.00+0     doi:10.1128/AEM.02273-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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