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Applied and Environmental Microbiology, April 2009, p. 2037-2045, Vol. 75, No. 7
0099-2240/09/$08.00+0     doi:10.1128/AEM.01686-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Unexpected Genetic Diversity among and within Populations of the Toxic Dinoflagellate Alexandrium catenella as Revealed by Nuclear Microsatellite Markers{triangledown}

Estelle Masseret,1* Daniel Grzebyk,1 Satoshi Nagai,2 Benjamin Genovesi,1,2 Bernard Lasserre,1 Mohamed Laabir,1 Yves Collos,1 André Vaquer,1 and Patrick Berrebi3

Université Montpellier II, CNRS, Ifremer, UMR 5119 Ecosystèmes Lagunaires 5119, cc093, Place E. Bataillon, 34095 Montpellier, France,1 National Research Institute of Fisheries and Environment of the Inland Sea, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan,2 Université Montpellier II, CNRS, UMR 5554, Institut des Sciences de l'Evolution, cc065, Place E. Bataillon, 34095 Montpellier, France3

Received 21 July 2008/ Accepted 6 January 2009

Since 1998, blooms of Alexandrium catenella associated with paralytic shellfish poisoning have been repeatedly reported for Thau Lagoon (French Mediterranean coast). Based on data obtained for rRNA gene markers, it has been suggested that the strains involved could be closely related to the Japanese temperate Asian ribotype of the temperate Asian clade. In order to gain more insight into the origin of these organisms, we carried out a genetic analysis of 61 Mediterranean and 23 Japanese strains using both ribosomal and microsatellite markers. Whereas the phylogeny based on ribosomal markers tended to confirm the previous findings, the analysis of microsatellite sequences revealed an unexpected distinction between the French and Japanese populations. This analysis also highlighted great intraspecific diversity that was not detected with the classical rRNA gene markers. The Japanese strains are divided into two differentiated A. catenella lineages: the Sea of Japan lineage and the east coast lineage, which includes populations from the Inland Sea and the Pacific Ocean. A. catenella strains isolated from Thau Lagoon belong to another lineage. These findings indicate that microsatellite markers are probably better suited to investigations of the population genetics of this species that is distributed worldwide. Finally, application of the population genetics concepts available for macroorganisms could support new paradigms for speciation and migration in phytoplankton assemblages.

* Corresponding author. Mailing address: UMR UM2-CNRS-Ifremer 5119 Ecosystèmes lagunaires, Université Montpellier II, cc093, Place Eugène Bataillon, 34095 Montpellier, France. Phone: 33 467144762. Fax: 33 467143719. E-mail: estelle.masseret{at}univ-montp2.fr

{triangledown} Published ahead of print on 5 February 2009.

Applied and Environmental Microbiology, April 2009, p. 2037-2045, Vol. 75, No. 7
0099-2240/09/$08.00+0     doi:10.1128/AEM.01686-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Genovesi, B., Laabir, M., Masseret, E., Collos, Y., Vaquer, A., Grzebyk, D. (2009). Dormancy and germination features in resting cysts of Alexandrium tamarense species complex (Dinophyceae) can facilitate bloom formation in a shallow lagoon (Thau, southern France). J PLANKTON RES 31: 1209-1224 [Abstract] [Full Text]  


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