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Applied and Environmental Microbiology, April 2009, p. 2074-2078, Vol. 75, No. 7
0099-2240/09/$08.00+0     doi:10.1128/AEM.02182-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection of Campylobacter Bacteria in Air Samples for Continuous Real-Time Monitoring of Campylobacter Colonization in Broiler Flocks{triangledown}

Katja N. Olsen,1,3 Marianne Lund,2 Julia Skov,3 Laurids S. Christensen,1* and Jeffrey Hoorfar1

National Food Institute, Technical University of Denmark, DK-2860 Søborg, Denmark,1 National Veterinary Institute, Technical University of Denmark, DK-8200 Århus N, Denmark,2 ilochip A/S, DK-9800 Nørresundby, Denmark3

Received 22 September 2008/ Accepted 1 February 2009

Improved monitoring tools are important for the control of Campylobacter bacteria in broiler production. In this study, we compare the sensitivities of detection of Campylobacter by PCR with feces, dust, and air samples during the lifetimes of broilers in two poultry houses and conclude that the sensitivity of detection of Campylobacter in air is comparable to that in other sample materials. Profiling of airborne particles in six poultry houses revealed that the aerodynamic conditions were dependent on the age of the chickens and very comparable among different poultry houses, with low proportions of particles in the 0.5- to 2-µm-diameter range and high proportions in the 2- to 5-µm-diameter range. Campylobacter could also be detected by PCR in air samples collected at the hanging stage during the slaughter process but not at the other stages tested at the slaughterhouse. The exploitation of airborne dust in poultry houses as a sample material for the detection of Campylobacter and other pathogens provides an intriguing possibility, in conjunction with new detection technologies, for allowing continuous or semicontinuous monitoring of colonization status.

* Corresponding author. Mailing address: National Food Institute, Technical University of Denmark, 19 Mørkhøj Bygade, Blok H, DK-2680 Søborg, Denmark. Phone: (45) 22844762. Fax: (45) 72347001. E-mail: lasi{at}food.dtu.dk

{triangledown} Published ahead of print on 6 February 2009.

Applied and Environmental Microbiology, April 2009, p. 2074-2078, Vol. 75, No. 7
0099-2240/09/$08.00+0     doi:10.1128/AEM.02182-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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