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Applied and Environmental Microbiology, April 2009, p. 2236-2237, Vol. 75, No. 7
0099-2240/09/$08.00+0     doi:10.1128/AEM.02342-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Interaction of Bacillus thuringiensis Cry1 and Vip3A Proteins with Spodoptera frugiperda Midgut Binding Sites{triangledown}

Janete A. D. Sena,{dagger} Carmen Sara Hernández-Rodríguez, and Juan Ferré*

Departamento de Genética, Facultad de CC. Biológicas, Universidad de Valencia, Valencia, Spain

Received 13 October 2008/ Accepted 17 January 2009

Vip3Aa, Vip3Af, Cry1Ab, and Cry1Fa were tested for their toxicities and binding interactions. Vip3A proteins were more toxic than Cry1 proteins. Binding assays showed independent specific binding sites for Cry1 and Vip3A proteins. Cry1Ab and Cry1Fa competed for the same binding sites, whereas Vip3Aa competed for those of Vip3Af.

* Corresponding author. Mailing address: Departamento de Genética, Facultad de CC. Biológicas, Dr. Moliner 50, 46100-Burjassot, Valencia, Spain. Phone: (34) 96 354 4506. Fax: (34) 96 354 3029. E-mail: juan.ferre{at}uv.es

{triangledown} Published ahead of print on 30 January 2009.

{dagger} Present address: Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Biologia Aplicada a Agropecuaria, Jaboticabal, São Paulo, Brazil.

Applied and Environmental Microbiology, April 2009, p. 2236-2237, Vol. 75, No. 7
0099-2240/09/$08.00+0     doi:10.1128/AEM.02342-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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