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Applied and Environmental Microbiology, April 2009, p. 2366-2374, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02479-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Cellulase Production from Spent Lignocellulose Hydrolysates by Recombinant Aspergillus niger{triangledown}

Björn Alriksson,1 Shaunita H. Rose,2 Willem H. van Zyl,2 Anders Sjöde,1 Nils-Olof Nilvebrant,3,{dagger} and Leif J. Jönsson1,4*

Department of Chemistry and Biomedical Sciences, Karlstad University, Karlstad, Sweden,1 Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa,2 STFI-Packforsk AB, Stockholm, Sweden,3 Department of Chemistry, Umeå University, Umeå, Sweden4

Received 29 October 2008/ Accepted 17 February 2009

A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content (e.g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in standard glucose-based medium). In addition, A. niger D15[egI] was also able to consume or convert other lignocellulose-derived compounds, such as acetic acid, furan aldehydes, and phenolic compounds, which are recognized as inhibitors of yeast during ethanolic fermentation. The results indicate that enzymes can be produced from the stillage stream as a high-value coproduct in second-generation bioethanol plants in a way that also facilitates recirculation of process water.

* Corresponding author. Mailing address: Department of Chemistry, Umeå University, SE-90187 Umeå, Sweden. Phone: 46-(0)90-7866811. Fax: 46-(0)90-7867655. E-mail: leif.jonsson{at}chem.umu.se

{triangledown} Published ahead of print on 27 February 2009.

{dagger} Present address: Department of Chemical Engineering, Karlstad University, Karlstad, Sweden.

Applied and Environmental Microbiology, April 2009, p. 2366-2374, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02479-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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