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Applied and Environmental Microbiology, April 2009, p. 2400-2405, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02112-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

A Novel Dehalobacter Species Is Involved in Extensive 4,5,6,7-Tetrachlorophthalide Dechlorination{triangledown}

Naoko Yoshida,* Lizhen Ye, Daisuke Baba, and Arata Katayama

EcoTopia Science Institute, Nagoya University, Nagoya 464-8603, Japan

Received 11 September 2008/ Accepted 6 February 2009

The purpose of this study was the enrichment and phylogenetic identification of bacteria that dechlorinate 4,5,6,7-tetrachlorophthalide (commercially designated "fthalide"), an effective fungicide for rice blast disease. Sequential transfer culture of a paddy soil with lactate and fthalide produced a soil-free enrichment culture (designated the "KFL culture") that dechlorinated fthalide by using hydrogen, which is produced from lactate. Phylogenetic analysis based on 16S rRNA genes revealed the dominance of two novel phylotypes of the genus Dehalobacter (FTH1 and FTH2) in the KFL culture. FTH1 and FTH2 disappeared during culture transfer in medium without fthalide and increased in abundance with the dechlorination of fthalide, indicating their growth dependence on the dechlorination of fthalide. Dehalobacter restrictus TEA is their closest relative, with 97.5% and 97.3% 16S rRNA gene similarities to FTH1 and FTH2, respectively.

* Corresponding author. Mailing address: EcoTopia Science Institute, Nagoya University, Nagoya 464-8603, Japan. Phone: 81 52 789 5841. Fax: 81 52 789 5857. E-mail: ysd75{at}esi.nagoya-u.ac.jp

{triangledown} Published ahead of print on 13 February 2009.

Applied and Environmental Microbiology, April 2009, p. 2400-2405, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02112-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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