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Applied and Environmental Microbiology, April 2009, p. 2534-2544, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02282-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzevii{triangledown} ,{dagger}

Carmela Belloch,1 Roberto Pérez-Torrado,1 Sara S. González,1 José E. Pérez-Ortín,2 José García-Martínez,2 Amparo Querol,1 and Eladio Barrio3*

Instituto de Agroquímica y Tecnología de los Alimentos, IATA-CSIC, P.O. Box 73, E-46100 Burjassot, Spain,1 Department de Bioquímica i Biologia Molecular, Facultat de Ciències Biològiques, Universitat de València, C/ Dr. Moliner 50, E-46100 Burjassot, Spain,2 Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, P.O. Box 22185, E-46071 València, Spain3

Received 4 October 2008/ Accepted 17 February 2009

Recently, a new type of hybrid resulting from the hybridization between Saccharomyces cerevisiae and Saccharomyces kudriavzevii was described. These strains exhibit physiological properties of potential biotechnological interest. A preliminary characterization of these hybrids showed a trend to reduce the S. kudriavzevii fraction of the hybrid genome. We characterized the genomic constitution of several wine S. cerevisiae x S. kudriavzevii strains by using a combined approach based on the restriction fragment length polymorphism analysis of gene regions, comparative genome hybridizations with S. cerevisiae DNA arrays, ploidy analysis, and gene dose determination by quantitative real-time PCR. The high similarity in the genome structures of the S. cerevisiae x S. kudriavzevii hybrids under study indicates that they originated from a single hybridization event. After hybridization, the hybrid genome underwent extensive chromosomal rearrangements, including chromosome losses and the generation of chimeric chromosomes by the nonreciprocal recombination between homeologous chromosomes. These nonreciprocal recombinations between homeologous chromosomes occurred in highly conserved regions, such as Ty long terminal repeats (LTRs), rRNA regions, and conserved protein-coding genes. This study supports the hypothesis that chimeric chromosomes may have been generated by a mechanism similar to the recombination-mediated chromosome loss acting during meiosis in Saccharomyces hybrids. As a result of the selective processes acting during fermentation, hybrid genomes maintained the S. cerevisiae genome but reduced the S. kudriavzevii fraction.

* Corresponding author. Mailing address: Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, P.O. Box 22185, E-46071 València, Spain. Phone: 34 963 543 667. Fax: 34 963 543 670. E-mail: eladio.barrio{at}uv.es

{triangledown} Published ahead of print on 27 February 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, April 2009, p. 2534-2544, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02282-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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