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Applied and Environmental Microbiology, April 2009, p. 2581-2584, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.01500-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals{triangledown} ,{dagger}

Raju Sekar,* Longin T. Kaczmarsky, and Laurie L. Richardson

Department of Biological Sciences, Florida International University, Miami, Florida 33199

Received 3 July 2008/ Accepted 18 February 2009

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

* Corresponding author. Present address: Biological and Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Mappin Street, Sheffield S1 3JD, United Kingdom. Phone: 44 114 222 0062. Fax: 44 114 222 7501. E-mail: S.Raju{at}Sheffield.ac.uk

{triangledown} Published ahead of print on 27 February 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, April 2009, p. 2581-2584, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.01500-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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