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Applied and Environmental Microbiology, April 2009, p. 2598-2602, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02436-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Community Composition and Density of Methanogens in the Foregut of the Tammar Wallaby (Macropus eugenii){triangledown}

Paul N. Evans,1* Lyn A. Hinds,2 Lindsay I. Sly,3 Christopher S. McSweeney,1 Mark Morrison,1 and André-Denis G. Wright1

CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Brisbane, Queensland 4067, Australia,1 CSIRO Entomology, GPO Box 1700, Canberra, Australian Capital Territory 2601, Australia,2 School of Chemistry and Molecular Biosciences, University of Queensland, St. Lucia, Brisbane, Queensland 4067, Australia3

Received 23 October 2008/ Accepted 2 February 2009

The composition of the methanogenic archaeal community in the foregut contents of Tammar wallabies (Macropus eugenii) was studied using 16S rRNA and methyl coenzyme reductase subunit A (mcrA) gene clone libraries. Methanogens belonging to the Methanobacteriales and a well-supported cluster of uncultivated archaeon sequences previously observed in the ovine and bovine rumens were found. Methanogen densities ranged from 7.0 x 105 and 3.9 x 106 cells per gram of wet weight.

* Corresponding author. Mailing address: CSIRO Livestock Industries, Queensland Bioscience Precinct, 306 Carmody Road, St. Lucia, Brisbane, Queensland 4067, Australia. Phone: 61 7 3214 2200. Fax: 61 7 3214 2900. E-mail: Paul.Evans{at}csiro.au

{triangledown} Published ahead of print on 13 February 2009.

Applied and Environmental Microbiology, April 2009, p. 2598-2602, Vol. 75, No. 8
0099-2240/09/$08.00+0     doi:10.1128/AEM.02436-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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