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Applied and Environmental Microbiology, May 2009, p. 2659-2667, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02483-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of a New Plasmid-Like Prophage in a Pandemic Vibrio parahaemolyticus O3:K6 Strain {triangledown} ,{dagger}

Shih-Feng Lan,1 Chung-Ho Huang,1 Chuan-Hsiung Chang,2 Wei-Chao Liao,3 I-Hsuan Lin,2 Wan-Neng Jian,4 Yueh-Gin Wu,1 Shau-Yan Chen,1 and Hin-chung Wong1*

Department of Microbiology, Soochow University, Taipei, Taiwan 111, Republic of China,1 Institute of BioMedical Informatics, National Yang-Ming University, Taipei, Taiwan 112, Republic of China,2 Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan 112, Republic of China,3 Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China4

Received 29 October 2008/ Accepted 6 March 2009

Vibrio parahaemolyticus is a common food-borne pathogen that is normally associated with seafood. In 1996, a pandemic O3:K6 strain abruptly appeared and caused the first pandemic of this pathogen to spread throughout many Asian countries, America, Europe, and Africa. The role of temperate bacteriophages in the evolution of this pathogen is of great interest. In this work, a new temperate phage, VP882, from a pandemic O3:K6 strain of V. parahaemolyticus was purified and characterized after mitomycin C induction. VP882 was a Myoviridae bacteriophage with a polyhedral head and a long rigid tail with a sheath-like structure. It infected and lysed high proportions of V. parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae strains. The genome of phage VP882 was sequenced and was 38,197 bp long, and 71 putative open reading frames were identified, of which 27 were putative functional phage or bacterial genes. VP882 had a linear plasmid-like genome with a putative protelomerase gene and cohesive ends. The genome does not integrate into the host chromosome but was maintained as a plasmid in the lysogen. Analysis of the reaction sites of the protelomerases in different plasmid-like phages revealed that VP882 and {Phi}HAP-1 were highly similar, while N15, {Phi}KO2, and PY54 made up another closely related group. The presence of DNA adenine methylase and quorum-sensing transcriptional regulators in VP882 may play a specific role in this phage or regulate physiological or virulence-associated traits of the hosts. These genes may also be remnants from the bacterial chromosome following transduction.

* Corresponding author. Mailing address: Department of Microbiology, Soochow University, 70 Lin-Si Rd., Taipei, Taiwan 111, Republic of China. Phone: 886-2-28819471, ext. 6852. Fax: 886-2-28831193. E-mail: wonghc{at}scu.edu.tw

{triangledown} Published ahead of print on 13 March 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2009, p. 2659-2667, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02483-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zabala, B., Hammerl, J. A., Espejo, R. T., Hertwig, S. (2009). The Linear Plasmid Prophage Vp58.5 of Vibrio parahaemolyticus Is Closely Related to the Integrating Phage VHML and Constitutes a New Incompatibility Group of Telomere Phages. J. Virol. 83: 9313-9320 [Abstract] [Full Text]  


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