This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sharp, K.
Right arrow Articles by Paul, V. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sharp, K.
Right arrow Articles by Paul, V. J.
Agricola
Right arrow Articles by Sharp, K.
Right arrow Articles by Paul, V. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2009, p. 2879-2888, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02656-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Phylogenetic and Chemical Diversity of Three Chemotypes of Bloom-Forming Lyngbya Species (Cyanobacteria: Oscillatoriales) from Reefs of Southeastern Florida{triangledown}

Koty Sharp,1,{dagger} Karen E. Arthur,1 Liangcai Gu,2 Cliff Ross,3 Genelle Harrison,1 Sarath P. Gunasekera,1 Theresa Meickle,1 Susan Matthew,4 Hendrik Luesch,4 Robert W. Thacker,5 David H. Sherman,2 and Valerie J. Paul1*

Smithsonian Marine Station at Fort Pierce, 701 Seaway Drive, Fort Pierce, Florida 34949,1 Life Sciences Institute and Departments of Medicinal Chemistry, Chemistry, and Microbiology & Immunology, University of Michigan, 210 Washtenaw Ave., Ann Arbor, Michigan 48109,2 University of North Florida, 1 UNF Drive, Jacksonville, Florida 32224,3 Department of Medicinal Chemistry, University of Florida, 1600 SW Archer Road, Gainesville, Florida 32610,4 Department of Biology, University of Alabama at Birmingham, Birmingham, Alabama 35294-11705

Received 19 November 2008/ Accepted 26 February 2009

The cyanobacterial genus Lyngbya includes free-living, benthic, filamentous cyanobacteria that form periodic nuisance blooms in lagoons, reefs, and estuaries. Lyngbya spp. are prolific producers of biologically active compounds that deter grazers and help blooms persist in the marine environment. Here, our investigations reveal the presence of three distinct Lyngbya species on nearshore reefs in Broward County, FL, sampled in 2006 and 2007. With a combination of morphological measurements, molecular biology techniques, and natural products chemistry, we associated these three Lyngbya species with three distinct Lyngbya chemotypes. One species, identified as Lyngbya cf. confervoides via morphological measurements and 16S rRNA gene sequencing, produces a diverse array of bioactive peptides and depsipeptides. Our results indicate that the other two Lyngbya species produce either microcolins A and B or curacin D and dragonamides C and D. Results from screening for the biosynthetic capacity for curacin production among the three Lyngbya chemotypes in this study correlated that capacity with the presence of curacin D. Our work on these bloom-forming Lyngbya species emphasizes the significant phylogenetic and chemical diversity of the marine cyanobacteria on southern Florida reefs and identifies some of the genetic components of those differences.

* Corresponding author. Mailing address: Smithsonian Marine Station at Fort Pierce, 701 Seaway Drive, Fort Pierce, FL 34949. Phone: (772) 462-0982. Fax: (772) 461-8154. E-mail: paul{at}si.edu

{triangledown} Published ahead of print on 6 March 2009.

{dagger} Present address: Ocean Genome Legacy Foundation, New England Biolabs, 240 County Rd., Ipswich, MA 01938.

Applied and Environmental Microbiology, May 2009, p. 2879-2888, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02656-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»