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Applied and Environmental Microbiology, May 2009, p. 2936-2939, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02524-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

PCR-Based Method Using Propidium Monoazide To Distinguish Viable from Nonviable Bacillus subtilis Spores{triangledown}

H. Rawsthorne,* C. N. Dock,{dagger} and L. A. Jaykus

Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina 27695-7624

Received 4 November 2008/ Accepted 25 February 2009

This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores.

* Corresponding author. Mailing address: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, 400 Dan Allen Drive, Raleigh, NC 27695-7624. Phone: (919) 515-3558. Fax: (919) 513-0014. E-mail: Helen_Rawsthorne{at}NCSU.edu

{triangledown} Published ahead of print on 6 March 2009.

{dagger} Present address: California Institute of Technology, Jet Propulsion Laboratory, Biotechnology and Planetary Protection Group, M/S 89-3, 4800 Oak Grove Dr., Pasadena, CA 91109.

Applied and Environmental Microbiology, May 2009, p. 2936-2939, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02524-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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