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Applied and Environmental Microbiology, May 2009, p. 2940-2944, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.01333-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Discrimination of Viable and Dead Fecal Bacteroidales Bacteria by Quantitative PCR with Propidium Monoazide{triangledown}

Sungwoo Bae and Stefan Wuertz*

Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, California 95616

Received 13 June 2008/ Accepted 23 February 2009

Propidium monoazide (PMA) was optimized to discriminate between viable and dead Bacteroides fragilis cells and extracellular DNA at different concentrations of solids using quantitative PCR. Conditions of 100 µM PMA and a 10-min light exposure also excluded DNA from heat-treated cells of nonculturable Bacteroidales in human feces and wastewater influent and effluent.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, 2001 EU III, University of California, Davis, One Shields Avenue, Davis, CA 95616. Phone: (530) 754-6407. Fax: (530) 752-7872. E-mail: swuertz{at}ucdavis.edu

{triangledown} Published ahead of print on 6 March 2009.

Applied and Environmental Microbiology, May 2009, p. 2940-2944, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.01333-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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