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Applied and Environmental Microbiology, May 2009, p. 2945-2950, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02610-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Panel of 23S rRNA Gene-Based Real-Time PCR Assays for Improved Universal and Group-Specific Detection of Phytoplasmas{triangledown} ,{dagger}

Jennifer Hodgetts,1 Neil Boonham,2 Rick Mumford,2 and Matthew Dickinson1*

School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom,1 Central Science Laboratory, Sand Hutton, York YO41 1LZ, United Kingdom2

Received 14 November 2008/ Accepted 25 February 2009

Primers and probes based on the 23S rRNA gene have been utilized to design a range of real-time PCR assays for routine phytoplasma diagnostics. These assays have been authenticated as phytoplasma specific and shown to be at least as sensitive as nested PCR. A universal assay to detect all phytoplasmas has been developed, along with a multiplex assay to discriminate 16SrI group phytoplasmas from members of all of the other 16Sr groups. Assays for the 16SrII, 16SrIV, and 16SrXII groups have also been developed to confirm that the 23S rRNA gene can be used to design group-specific assays.

* Corresponding author. Mailing address: School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom. Phone: 0115 9513236. Fax: 0115 9516334. E-mail: matthew.dickinson{at}nottingham.ac.uk

{triangledown} Published ahead of print on 6 March 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2009, p. 2945-2950, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02610-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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