This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Simon, C.
Right arrow Articles by Daniel, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Simon, C.
Right arrow Articles by Daniel, R.
Agricola
Right arrow Articles by Simon, C.
Right arrow Articles by Daniel, R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2009, p. 2964-2968, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice{triangledown}

Carola Simon, Judith Herath, Stephanie Rockstroh, and Rolf Daniel*

Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstrasse 8, 37077 Göttingen, Germany

Received 18 November 2008/ Accepted 1 March 2009

Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Genetik der Georg-August-Universität, Grisebachstr. 8, 37077 Göttingen, Germany. Phone: 49-551-393827. Fax: 49-551-3912181. E-mail: rdaniel{at}gwdg.de

{triangledown} Published ahead of print on 6 March 2009.

Applied and Environmental Microbiology, May 2009, p. 2964-2968, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Simon, C., Wiezer, A., Strittmatter, A. W., Daniel, R. (2009). Phylogenetic Diversity and Metabolic Potential Revealed in a Glacier Ice Metagenome. Appl. Environ. Microbiol. 75: 7519-7526 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»