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Applied and Environmental Microbiology, May 2009, p. 2964-2968, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Genes Encoding DNA Polymerases by Function-Based Screening of Metagenomic Libraries Derived from Glacial Ice{triangledown}

Carola Simon, Judith Herath, Stephanie Rockstroh, and Rolf Daniel*

Abteilung Genomische und Angewandte Mikrobiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität, Grisebachstrasse 8, 37077 Göttingen, Germany

Received 18 November 2008/ Accepted 1 March 2009

Small-insert and large-insert metagenomic libraries were constructed from glacial ice of the Northern Schneeferner, which is located on the Zugspitzplatt in Germany. Subsequently, these libraries were screened for the presence of DNA polymerase-encoding genes by complementation of an Escherichia coli polA mutant. Nine novel genes encoding complete DNA polymerase I proteins or domains typical of these proteins were recovered.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Genetik der Georg-August-Universität, Grisebachstr. 8, 37077 Göttingen, Germany. Phone: 49-551-393827. Fax: 49-551-3912181. E-mail: rdaniel{at}gwdg.de

{triangledown} Published ahead of print on 6 March 2009.

Applied and Environmental Microbiology, May 2009, p. 2964-2968, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02644-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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