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Applied and Environmental Microbiology, May 2009, p. 2969-2972, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02051-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

TaqMan Probe-Based Real-Time PCR Assay for Detection and Discrimination of Class I, II, and III tfdA Genes in Soils Treated with Phenoxy Acid Herbicides{triangledown} ,{dagger}

Jacob Bælum1 and Carsten S. Jacobsen1,2*

Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark,1 Faculty of Life Sciences, Department of Natural Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark2

Received 4 September 2008/ Accepted 19 February 2009

Separate quantification of three classes of tfdA genes was performed using TaqMan quantitative real-time PCR for 13 different soils subsequent to mineralization of three phenoxy acids. Class III tfdA genes were found to be involved in mineralization more often than class I and II tfdA genes.

* Corresponding author. Mailing address: Geological Survey of Denmark and Greenland (GEUS), Department of Geochemistry, Øster Voldgade 10, DK-1350 Copenhagen K, Denmark. Phone: 45 3814 2313. Fax: 45 3814 2050. E-mail: csj{at}geus.dk

{triangledown} Published ahead of print on 27 February 2009.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, May 2009, p. 2969-2972, Vol. 75, No. 9
0099-2240/09/$08.00+0     doi:10.1128/AEM.02051-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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