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Applied and Environmental Microbiology, February 2010, p. 751-756, Vol. 76, No. 3
0099-2240/10/$12.00+0     doi:10.1128/AEM.02230-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Inhibitory Effects of Low-Energy Pulsed Ultrasonic Stimulation on Cell Surface Protein Antigen C through Heat Shock Proteins GroEL and DnaK in Streptococcus mutans{triangledown}

Kazuya Ishibashi,1 Koichi Shimada,2,4* Takayuki Kawato,3,5 Shigejyu Kaji,6 Masao Maeno,3,5 Shuichi Sato,2,4 and Koichi Ito2,4

Division of Applied Oral Sciences, Nihon University Graduate School of Dentistry,1 Departments of Periodontology,2 Oral Health Sciences,3 Divisions of Advanced Dental Treatment,4 Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan,5 Asahi Irika Company Ltd., Saitama, Japan6

Received 9 September 2009/ Accepted 5 December 2009

This study concerns the use of low-energy pulsed ultrasound as nondestructive photodynamic antimicrobial therapy for controlling dental plaque. We examined the antibacterial and bactericidal effects of low-energy pulsed ultrasound on mutans streptococci and its inhibitory effects on bacterial cell adhesion of Streptococcus mutans. The results indicated weak antibacterial and bactericidal effects. However, ultrasonic stimulation for less than 20 min markedly decreased bacterial cell adhesion. To analyze the mechanism underlying the inhibitory effect, we examined cell surface protein antigen C (PAc) and glucosyltransferase I (GTF-I) expression in S. mutans. The levels of PAc gene and protein expression were markedly decreased by ultrasonic stimulation for 20 min. However, no change in GTF-I expression was observed. The expression of stress response heat shock proteins GroEL and DnaK was also examined. GroEL and DnaK levels were significantly decreased by ultrasonic stimulation, and the expression of the PAc protein was also diminished upon the addition of GroEL or DnaK inhibitors without ultrasonic stimulation. These observations suggest that the expression of the PAc protein in S. mutans may be dependent on heat shock proteins. Thus, low-energy pulsed ultrasound decreases bacterial adhesion by the inhibitory effect on the PAc protein and heat shock protein expression and may be useful as photodynamic antimicrobial chemotherapy in controlling dental plaque.

* Corresponding author. Mailing address: 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan. Phone: 81-3-3219-8107. Fax: 81-3-3219-8349. E-mail: shimada-ki{at}dent.nihon-u.ac.jp

{triangledown} Published ahead of print on 18 December 2009.

Applied and Environmental Microbiology, February 2010, p. 751-756, Vol. 76, No. 3
0099-2240/10/$12.00+0     doi:10.1128/AEM.02230-09
Copyright © 2010, American Society for Microbiology. All Rights Reserved.





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