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Appl Environ Microbiol. 1961 July; 9(4): 303-308

Studies on Media for Enumerating Enterococci in Frozen Vegetables¹

New York State Agricultural Experiment Station, Cornell University, Geneva, New York

ABSTRACT

A study was made of the relative sensitivity and specificity of presumptive and confirmatory media for the most probable number enumeration of enterococci in frozen vegetables. Azide dextrose broth yielded the highest numbers of confirmable enterococci and its sensitivity was shown to be comparable to nonselective media. The use of ethyl violet azide broth as a confirmatory medium resulted in a significant number of false positive tests. Growth in broth containing 6.5% sodium chloride incubated at 45 C for 2 days was found to be a more specific confirmatory test for enterococci.

¹ Approved by the Director of the New York State Agricultural Experiment Station for publication as Journal paper no. 1237, October 4, 1960.