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Appl Environ Microbiol. 1961 November; 9(6): 469-471

Purity Control of the Production of Baker's Yeast Employing a Fluorescent Antiserum

Ch. Kunz and H. Klaushofer

Hygiene-Institut der Universität Wien, Institut für Angewandte Mikrobiologie der Hochschule für Bodenkultur, Wien, Austria

ABSTRACT

A simple staining procedure for the rapid detection of wild yeasts contaminating baker's yeast during the course of industrial production is described. Fluorescein-labeled, specific antiserum against Saccharomyces cerevisiae is applied to smears of baker's yeast which are then examined by ultraviolet microscopy. Optimal results are obtained with the combined phase contrast and fluorescence which makes the S. cerevisiae appear green, whereas cells of wild yeasts are visible in bright red counterstain. With this method, wild yeasts could be identified in baker's yeast at a dilution of 1:10,000.

Appl Environ Microbiol. 1961 November; 9(6): 469-471






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