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AEM Accepts, published online ahead of print on 2 May 2008
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Appl. Environ. Microbiol. doi:10.1128/AEM.00127-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Rapid and Sensitive Detection of Norovirus Genomes in Oysters by a Two-Step Isothermal Amplification assay system combining the Nucleic Acid Sequence-based Amplification (NASBA) and Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) assays

Shinji Fukuda*, Yukie Sasaki, and Masato Seno

Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Hiroshima 734-0007, Japan

* To whom correspondence should be addressed. Email: s-fukuda80723{at}pref.hiroshima.lg.jp.


  Abstract

We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification time of NoV genomes from RNA extracts was shortened to about 3 h.




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