AEM Accepts, published online ahead of print on 5 January 2009
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Appl. Environ. Microbiol. doi:10.1128/AEM.01944-08
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Tape-FISH for Salmonella: Simple Adhesive Tape-Based Sampling of Tomato Surfaces Combined with Rapid Whole Cell Hybridization

Bledar Bisha and Byron F. Brehm-Stecher*

Rapid Microbial Detection and Control Laboratory, Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011

* To whom correspondence should be addressed. Email: byron{at}iastate.edu.


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Abstract

A simple adhesive tape-based method for sampling of tomato surfaces was combined with fluorescence in situ hybridization (FISH) for rapid culture-independent detection of Salmonella spp. Tapes could also be placed face-down on selective agar for on-tape enrichment of captured Salmonella cells. Overlay of cell-charged tapes with small volumes of liquid enrichment media enabled subsequent detection of tape-captured Salmonella via flow cytometry.






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