AEM Accepts, published online ahead of print on 13 March 2009

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Appl. Environ. Microbiol. doi:10.1128/AEM.02108-08
Copyright (c) 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Antibody-recognition force microscopy shows that outer membrane cytochromes OmcA and MtrC are expressed on the exterior surface of Shewanella oneidensis MR-1

Brian H. Lower^*, Ruchirej Yongsunthon, Liang Shi, Linda Wildling, Hermann J. Gruber, Nicholas S. Wigginton, Catherine L. Reardon, Grigoriy E. Pinchuk, Timothy C. Droubay, Jean-François Boily, and Steven K. Lower

The Ohio State University, Columbus, Ohio 43210, USA; Corning Incorporated, Corning, New York 14831, USA; Pacific Northwest National Laboratory, Richland, Washington 99352, USA; Johannes Kepler University of Linz, A-4040 Linz, Austria; Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland; Umeå University, SE-901 87 Umeå, Sweden

^* To whom correspondence should be addressed. Email: Lower.30{at}osu.edu.

Antibody-recognition force microscopy showed that OmcA and MtrC are expressed on the exterior surface of living Shewanella oneidensis MR-1 cells when Fe(III), including solid phase hematite (Fe₂O₃), was the terminal electron acceptor. OmcA was localized to the interface between the cell and mineral. MtrC displayed a more uniform distribution across the cell surface. Both cytochromes were associated with an extracellular polymeric substance.