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Appl. Environ. Microbiol. doi:10.1128/AEM.02545-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Use of laser microdissection for phylogenetic characterization of polyphosphate-accumulating bacteria

Stefanie Gloess, Hans-Peter Grossart, Martin Allgaier, Stefan Ratering*, and Michael Hupfer*

Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, D-16775 Stechlin-Neuglobsow, Germany; Institute of Applied Microbiology of Justus-Liebig University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany

* To whom correspondence should be addressed. Email: Stefan.Ratering{at}agrar.uni-giessen.de. hupfer{at}igb-berlin.de.


  Abstract

Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.




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