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Appl Environ Microbiol, May 1998, p. 1731-1735, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Biodegradation of Aliphatic-Aromatic Copolyesters by Thermomonospora fusca and Other Thermophilic Compost Isolates

Ilona Kleeberg,¹ Claudia Hetz,¹ Reiner Michael Kroppenstedt,² Rolf-Joachim Müller,^1,* and Wolf-Dieter Deckwer¹

Gesellschaft für Biotechnologische Forschung mbH¹ and Deutsche Sammlung für Mikroorganismen und Zellkulturen,² D-38124 Braunschweig, Germany

Received 6 October 1997/Accepted 6 March 1998

	ABSTRACT

Top Abstract Introduction Materials & Methods Results & Discussion References

Random aliphatic-aromatic copolyesters synthesized from 1,4-butanediol, adipic acid, and terephthalic acid (BTA) have excellent thermal and mechanical properties and are biodegradable by mixed cultures (e.g., in compost). Over 20 BTA-degrading strains were isolated by using compost as a microbial source. Among these microorganisms, thermophilic actinomycetes obviously play an outstanding role and appear to dominate the initial degradation step. Two actinomycete strains exhibited about 20-fold higher BTA degradation rates than usually observed in a common compost test. These isolates were identified as Thermomonospora fusca strains. They appeared to be particularly suitable for establishment of rapid degradation tests and were used in comparative studies on the biodegradation of various polyesters.

	INTRODUCTION

Top Abstract Introduction Materials & Methods Results & Discussion References

Polymers designed to undergo controlled biological degradation are increasingly discussed as a favorable contribution to the solution of problems arising from plastic waste disposal, e.g., by recycling or land filling. The environmental safety of these novel materials has to be proven carefully (32). A number of standardized test methods using mixed cultures for evaluation of the biodegradability and compostability of plastics have been established recently (1, 5, 25, 30). However, for investigations of the degradation mechanism, it is advantageous to isolate individual strains which are able to degrade well-defined polymers.

For some polymers, including isoprene rubbers (7, 16, 34), polyvinyl alcohol (29), cellulose acetate (23), poly(-polycaprolactone) (24), and bacterial polyhydroxyalkanoates (6), a number of degrading microorganisms are described in the literature.

Recently, it has been shown that copolyesters containing adipic acid and terephthalic acid as aromatic acid components are also attacked by microorganisms (35). This group of copolyesters appears to be very promising with regard to widespread commercial applications (38). Studies have been performed with the particular goal of detecting the fate of the aromatic constituents and proving their biodegradation (36, 37).

However, in all previous investigations, inocula of undefined mixed cultures were used. Therefore, this study concentrated on the isolation of individual strains which are able to degrade random aliphatic-aromatic copolyesters. As composting is the most promising method of treating such biodegradable plastics, the microbial isolates were obtained from compost material. The isolates are appropriate candidates for use in the study of the mechanism of copolyester degradation and the establishment of improved and rapid test methods for evaluation of biodegradability.

	MATERIALS AND METHODS

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Polymers. A copolyester made of 1,4-butanediol, terephthalic acid, and adipic acid (Fig. 1) was used to isolate degradative microorganisms. The origins, compositions, melting temperatures, and average molar masses of all of the polymers tested in the biodegradation studies are listed in Table 1. Polymer films 100 µm thick and 25 mm in diameter were prepared as described by Witt et al. (35). The films were washed in a 70% (vol/vol) ethanol solution for 30 min, dried at room temperature under a vacuum, and weighed to an accuracy of ±0.2 mg. For sterilization, polymer films were irradiated under a UV lamp (UVC 30; Hereaus, Hannover, Germany; 254 nm, 6 W/cm² at a distance of 20 cm) at a lamp-to-film distance of 15 cm and an irradiation area of 38 by 18 cm for 15 min of exposure per side.

View larger version (10K): [in this window] [in a new window]   FIG. 1.   Formula of the aliphatic-aromatic copolyester BTA 40:60 used for the screening of microorganisms with regard to their degradation abilities. The copolyester consists of 1,4-butanediol, terephthalic acid, and adipic acid.

View this table: [in this window] [in a new window]   TABLE 1.   Compositions, melting temperatures, and average molar masses of tested polymers

Source of organisms. Aerobic microorganisms were isolated from samples of approximately 6-month-old mature compost made from green waste (Compost Plant Watenbüttel, Watenbüttel, Germany).

Media and buffer. The compositions of the media used are listed in Table 2 (3a, 4, 14). To retard drying of the agar media at high temperatures, petri dishes were incubated in sealed polyethylene bags (18). For preparation of suspensions and dilutions, a phosphate buffer (0.05 M Na₂HPO₄, 0.03 M KH₂PO₄, pH 7.0) was used.

View this table: [in this window] [in a new window]   TABLE 2.   Cultivation media

Isolation of BTA-degrading microorganisms. An at least four-step procedure turned out to be the appropriate strategy for the isolation of BTA-degrading strains. Mixed populations were used as inocula. These were taken by either (i) scraping off and dissolving (2 ml of phosphate buffer) preadapted biofilms grown on BTA films which were incubated in compost reactors as described by Witt et al. (35) or (ii) dissolving 10 g of mature compost or compost harvested from reactors in 90 ml of phosphate buffer.

For enrichment of BTA-degrading microorganisms on agar plates, BTA films were inoculated with 100 µl of the inocula mentioned above. Different mineral salt and compost extract agar plates were used as enrichment media (Table 2). The enrichment cultures were incubated at 20, 40, and 55°C and examined daily for growth of colonies and visible disintegration of the polymer films.

For isolation, colonies were picked up from partly disintegrated areas of films and cultured on various complex agar media (Table 2) by using different inoculation techniques to provide favorable growth conditions for a wide range of microorganisms.

We could not produce homogeneous opaque top layer agar plates with granules of BTA (aggregation of particles due to the hydrophobic surfaces). Thus, direct selection of degrading microorganisms by a clear-zone method (2) was not possible. Because of these problems, the abilities of all isolates to degrade the copolyester BTA 40:60 had to be tested in a separate step.

The degradation test was carried out with polymer films on three mineral salt media and one compost extract medium (Table 2) on agar plates inoculated with actinospore or bacterial suspensions (10⁷ microorganisms/ml) at the optimal growth temperature of the isolates. As an indicator for degradation, the weight loss of the polymer films was determined after 14 days of incubation. The results were compared with those obtained with noninoculated films (noninoculated controls) incubated in the respective media. The noninoculated controls showed no weight loss.

Cultivation and preservation of isolated strains. The isolates were maintained on agar plates of complex media at temperatures satisfying the individual requirements for optimal growth (Table 3). Bacterial strains were preserved in 50% (vol/vol) glycerol at 20°C. For recultivation, 1 loopful of each glycerol suspension was streaked onto an NB agar plate (Table 2). After 48 h of incubation, single colonies were picked and suspended in 1 ml of phosphate buffer. These bacterial suspensions served as the inoculum for degradation tests.

View this table: [in this window] [in a new window]   TABLE 3.   Incubation temperatures and media for cultivation and testing of BTA-degrading isolates

For preservation of the isolated actinomycete strains, spores from well-sporulating actinomycete cultures were suspended in distilled water and kept at 4°C in screw-cap tubes. Spore suspensions routinely served as an inoculum source for degradation tests.

Determination of the optimum growth temperatures of the individual strains was carried out on sections of agar plates inoculated with 1 loopful of an actinospore or bacterial suspension and incubated at temperatures of 20 to 70°C. Aerial mycelium formation and/or colony growth was visually observed after 1 and 2 weeks. All further tests were carried out at the respective optimum temperature.

For chemotaxonomic analysis, actinomycete cells were grown in TSB shake flask cultures, harvested by centrifugation (20 min, 7,800 × g, 4°C), washed twice in phosphate buffer, and freeze-dried to provide cell preparations (exception: for analysis of fatty acids, wet cells were used).

Taxonomic studies. For 16S ribosomal DNA (rDNA) sequencing, the genomic DNAs of K13g and K7a-3 were extracted and the 16S rRNA gene was amplified by PCR as described previously (27). Purified PCR products were directly sequenced by using the Taq Dye Deoxy Terminator Cycle Sequencing Kit (Applied Biosystems, Inc., Foster City, Calif.). Sequences were manually aligned with representatives of related sporoactinomycete taxa by using the ae2 editor available from the Ribosomal Database Project (17). The diagnostic regions of the 16S rDNA sequences were compared with those of the Thermomonospora type strains (28). Direct sequence similarities were calculated within the ae2 editor.

Analysis of cell wall amino acids and sugars. Amino acid and sugar analysis of the whole-cell hydrolysate was done as described by Staneck and Roberts (31).

Extraction and analysis of isoprenoid quinones and polar lipids. Isoprenoid quinones and polar lipids were extracted and purified by the small-scale integrated procedure of Minnikin et al. (21). The dried preparations of the quinone extracts were dissolved in 200 µl of isopropanol, and 1- to 10-µl amounts were separated by high-pressure liquid chromatography. The menaquinones were separated by high-pressure liquid chromatography on Lichrosorb RP-18 at 40°C using acetonitrile-isopropanol (65:35, vol/vol) as the solvent (10, 11). Polar lipid extracts were separated by two-dimensional thin-layer chromatography and identified by their R_f values and their reaction with diagnostic spray reagents (21).

Extraction and analysis of fatty acids. Fatty acid methyl esters were obtained from 40 mg of wet cells by saponification, methylation, and extraction by using minor modifications (15) of the method of Miller (20). The fatty acid methyl ester mixtures were separated by using a 5898A Microbial Identification System (Microbial ID, Newark, Del.). Peaks were automatically integrated and fatty acids were identified by the Microbial Identification System Standard Software (Microbial ID).

	RESULTS AND DISCUSSION

Top Abstract Introduction Materials & Methods Results & Discussion References

Screening and isolation of microorganisms. Although it was expected that BTA-degrading microorganisms would be enriched in biofilms grown on BTA films during composting, there was no significant difference in the number of BTA-degrading strains isolated from compost eluates or preadapted biofilms.

Table 3 gives the isolates, their optimal growth temperatures, and the media on which the isolates revealed the highest BTA degradation. As expected, different isolates showed maximal activities on different media, none of which generally appeared to be the best. Further degradation tests with individual strains were then carried out with the appropriate media. We obtained the best degradation results with temperatures higher than 40°C; thus, we focused predominantly on thermophilic microorganisms.

It must be admitted that weight loss measurements only indicate disintegration of the polymer films likely caused by cleavage of the polymer chains. Complete metabolism of the polymer material has to be investigated in additional tests. However, for polyesters, the first step in attacking the polymer chain is often the step which determines the degradability of such materials.

Table 4 gives an overview of the number of isolated bacteria, actinomycetes, and fungi and their abilities to degrade the aliphatic-aromatic copolyester BTA 40:60. A total of 61 strains of thermophilic microorganisms were isolated from 13 different compost samples.

View this table: [in this window] [in a new window]   TABLE 4.   Numbers of microorganisms isolated and their abilities to degrade the aliphatic-aromatic copolyester BTA 40:60a

Among 30 bacterial isolates, which were all aerobically growing rods mainly with endospore formation, only five strains were able to disintegrate BTA 40:60. Three of those strains exhibited only weak degradation activities (weight losses of 0.06 to 0.15 mg/week · cm²), just above the experimental error of the detection method used. Most fungi grow at temperatures of less than 50°C. This might be the reason why we isolated just two strains of fungi. Both strains were unable to disintegrate the copolyester BTA 40:60.

Within the group of thermophilic actinomycetes, only 4 of 29 isolates did not show significant degradation activities. Two of the most active actinomycetes, isolates K13g and K7a-3, were identified taxonomically and used for further investigations. They degraded films 100 µm thick up to 90% within 7 days.

More than 20 different microorganisms isolated from compost were able to depolymerize the synthetic polyester BTA 40:60. Probably due to the use of compost as the source of microorganisms, most isolates were thermophilic microorganisms. Especially, thermophilic actinomycetes play an outstanding role in degrading the BTA copolyester with regard to both the number of microorganisms isolated and their degradation rates. Actinomycetes are known to be involved in the degradation of several natural polymers, like chitin, celluloses, starch, agar, and lignocelluloses (3, 9, 18, 19). However, the role of this group of microorganisms in degrading synthetic polymers is rarely described in the literature (26).

Identification of selected strains. The phenotypic and chemotaxonomic properties of strains K13g and K7a-3 are consistent with their classification in the genus Thermomonospora (13). The strains showed generation of a white aerial mycelium and individual spores which were formed in clusters on the tip of a short sporophore. Both strains have the same chemotaxonomic characteristics which are consistent with members of the Thermomonospora fusca taxon (13). The whole-cell hydrolysates of strain K13g and K7a-3 contained glucose and ribose as major sugars. Mesodiaminopimelic acid was the only diamino acid found in the cell walls. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, methylphosphatidylethanolamine, and some unspecified glycolipids. The fatty acid pattern of the strains contained mainly iso- and anteiso-branched fatty acids. Small amounts of 10-methyl branched and unbranched fatty acids were also found.

MK-11(H₄), MK-11(H₆), MK-12(H₄), and MK-12(H₆) were the predominant menaquinones of both strains. The combination of chemical markers found in K13g and K7a-3 is unique to the strains of T. fusca. Therefore, K13g and K7a-3 could be classified as T. fusca by chemotaxonomy. This was confirmed by the analysis of 16S rDNA sequences of both isolates, which showed that isolates K7a-3 and K13g belong to the family Thermomonosporaceae. The greatest similarities were found between the isolates and T. fusca DSM 43792^T (K7a-3, 100%; K13g, 99.8%). Obviously, these two actinomycete isolates are representatives of this species.

BTA film degradation on agar plates by T. fusca K13g. With regard to the application of isolated strains as test organisms in improved degradation tests, the time course of the degradation of BTA films on agar plates by T. fusca K13g was investigated. Usually, enzymatic degradation of plastics is a surface erosion process, because enzymes are not able to penetrate the bulk polymer. Thus, the rate of weight loss can be directly used to measure the enzymatic cleavage of the polymer chains.

In Fig. 2, the weight loss of BTA films inoculated with a spore suspension of the actinomycete T. fusca K13g is plotted against the degradation time. A short period of only 7 days was sufficient to completely disintegrate the BTA films on the agar plates, resulting in a weight loss curve often observed for biological transformations. During a lag phase of 1 day, actinospores germinated; this was followed by a period of 3 days with constant weight loss of the exposed films. As the films started to fall into fragments, the degradation rate decreased gradually, and after 1 week, the BTA material disappeared totally. Although this high degree of degradation and the fact that the curve was obtained from films in parallel tests stopped at different times after incubation, the reproducibility of this experiment was very good.

View larger version (14K): [in this window] [in a new window]   FIG. 2.   Degradation of the aliphatic-aromatic copolyester BTA 40:60 on agar plates by the actinomycete T. fusca K13g. The test was carried out on MSV agar over a period of 13 days at 55°C with triplicate samples. From day 6 on, error bars are not shown because polymer films (diameter, 2.5 cm; thickness, 90 µm; initial weight, 46 mg; degradation area, 4.91 cm2) disappeared according to their initial weights (light films first) and error bars would represent the differences between the initial weights from that day on.

By calculating specific degradation rates from the linear part of the degradation curves in combination with the surface area of the films which could be attacked by the organisms, different experiments can be compared with regard to the degradation potential of the organisms involved.

Typical degradation rates of about 1 mg/week · cm² were obtained for BTA 40:60 films in a compost simulation test. Significantly higher degradation rates of up to 2.3 mg/week · cm² were found with preadapted mixed cultures from compost in an agar plate test. However, the increase in the degradation rate from compost to the preadapted mixed culture is not only related to the higher degradation potential of the organisms but also influenced by the optimized degradation conditions in the laboratory agar plate test. Remarkable degradation rates as high as 20 mg/week · cm² could be obtained with the actinomycete T. fusca K13g under defined laboratory conditions, which are about 10-fold higher than those achieved for the copolyester films incubated with adapted mixed cultures.

Compared to the fast degradation of T. fusca K13g, the degradation rate of bacterial strains K1a-1 and K1a-2 was quite slow. A continuous degradation of BTA films of only 0.1 mg/week · cm² within a test period of 6 weeks was observed.

The fast degradation of the BTA copolyester, in combination with the good reproducibility of the degradation rates in agar plate tests, predestine the actinomycete T. fusca K13g for use in rapid test methods and fundamental investigations of the degradation mechanism of the BTA copolyester and other polyesters as well.

Degradation of different kinds of polymers by two actinomycete isolates. The aromatic-aliphatic copolyester BTA 40:60 used for the screening is a biodegradable polymer of high commercial interest but does not occur in nature. Thus, to determine the substrate specificity of the isolated microorganisms, we tested the degradation of other polyesters and one polyester amide (Fig. 3) by T. fusca K13g and K7a-3. The two strains exhibited similarly high BTA 40:60 degradation abilities.

View larger version (29K): [in this window] [in a new window]   FIG. 3.   Degradation of different polyesters and the polyester amide Bayer Tir 1874 on agar plates by T. fusca K7a-3 and K13g. Polymer films (diameter, 2.5 cm; degradation area, 4.91 cm2) were incubated at 55°C for 2 weeks on MSV agar. A second test series gave reproducible results. Each series was carried out with triplicate samples. The degradation rate was calculated with data from the linear part of the degradation curve.

In all cases, the degradation rates obtained with strain K7a-3 were somewhat lower than those of strain K13g. Of the polyester-based materials tested, the aliphatic-aromatic copolyester BTA 40:60 exhibited the highest degradation rate, while pure aliphatic materials like Bionolle, Bayer Tir 1874, or even the bacterial polyester poly(-hydroxybutyrate) (PHB) were degraded much more slowly by both strains. This is surprising in that these polyesters are regarded to be easily biodegradable. Even a copolyester containing 60 mol% terephthalic acid disintegrates almost twice as fast as the natural material PHB. Polyesters with such a large aromatic compound fraction have been shown to degrade only very slowly in composting tests (35). The degradation behavior of SP313 is also unexpected. This aliphatic polyester with a long diacid component is not easily degraded in soil burial tests or in enzymatic degradation tests with lipases (22).

It can be anticipated that both thermophilic actinomycetes and their enzymatic systems prefer hydrophobic surfaces such as those offered by BTA copolyesters or the aliphatic polyester SP313. It will be challenging and illuminating to isolate and characterize the enzymatic system responsible for polymer chain cleavage and to compare its components with other polyester-degrading enzymes like lipases or PHB depolymerases (8, 33).

Despite the rapid depolymerization of the BTA copolyester, only poor growth of the actinomycetes could be observed on mineral salt agar with a polymer as the sole carbon source. This suggests that the actinomycetes are not able to completely metabolize the oligomers and monomers derived from the depolymerization of the polyester. As BTA copolyesters are abiotic synthetic materials, it is not likely that microorganisms have been specialized to this kind of carbon source. However, they are adapted to the use of other, similar polyester structures containing aromatic components like those in cutin or lignocelluloses.

Further investigations are in preparation to clarify the mechanism of BTA copolyester degradation by actinomycetes and to identify the enzymes involved.

	ACKNOWLEDGMENTS

We thank B. Frerichs, G. Pötter, I. Kramer, and J. Swiderski for their skilled technical assistance.

	FOOTNOTES

^* Corresponding author. Mailing address: Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, 38124 Braunschweig, Germany. Phone: 49 531 6181610. Fax: 49 531 6181175. E-mail: rmu{at}gbf.de.

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Appl Environ Microbiol, May 1998, p. 1731-1735, Vol. 64, No. 5
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