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Applied and Environmental Microbiology, February 1999, p. 724-731, Vol. 65, No. 2
National Institute of Agro-Environmental
Sciences, 3-1-1 Kan-nondai, Tsukuba, Ibaraki 305-8604, Japan
Received 10 August 1998/Accepted 17 November 1998
Alcaligenes eutrophus (Ralstonia eutropha)
NH9, isolated in Japan, utilizes 3-chlorobenzoate as its sole source of
carbon and energy. Sequencing of the relevant region of plasmid pENH91 from strain NH9 revealed that the genes for the catabolic enzymes were
homologous to the genes of the modified ortho-cleavage
pathway. The genes from strain NH9 (cbnR-ABCD) showed the
highest homology (89 to 100% identity at the nucleotide level) to the
tcbR-CDEF genes on plasmid pP51 of the
1,2,4-trichlorobenzene-degrading bacterium Pseudomonas sp.
strain P51, which was isolated in The Netherlands. The structure of the
operon, including the lengths of open reading frames and intervening
sequences, was completely conserved between the cbn and
tcb genes. Most nucleotide substitutions were
localized within and proximal to the cbnB
(tcbD) gene. The difference in the chloroaromatics
that the two strains could use as growth substrates seemed to be due to
differences in enzymes that convert substrates to chlorocatechols. The
restriction map of plasmid pENH91 was clearly different from that of
pP51 except in the regions that contained the cbnR-ABCD and
tcbR-CDEF genes, respectively, suggesting that the
chlorocatechol gene clusters might have been transferred as units. Two
homologous sequences, present as direct repeats in both flanking
regions of the cbnR-ABCD genes on pENH91, were found to be
identical insertion sequences (ISs), designated
IS1600, which formed a composite transposon designated
Tn5707. Although the tcbR-CDEF genes
were not associated with similar ISs, a DNA fragment homologous to
IS1600 was cloned from the chromosome of strain P51. The
sequence of the fragment suggested that it might be a remnant of an IS.
The two sequences, together with IS1326 and
nmoT, formed a distinct cluster on a phylogenetic tree of
the IS21 family. The diversity of the sources of
these IS or IS-like elements suggests the prevalence of ISs of this type.
Contamination with recalcitrant
chlorinated aromatic compounds is a very serious environmental problem
(3). In the most common examples of bacterial aerobic
degradation of chlorinated aromatics, the modified
ortho-cleavage pathway plays a significant role,
participating in the complete degradation of chlorocatechols that have
been generated from various chlorinated aromatics through convergent
pathways (61).
Three evolutionarily related clusters of genes of gram-negative
bacteria that encode enzymes in the modified ortho-cleavage pathway have been well described (for reviews, see references 14, 26, 55, and 61). These are
clcABD, tfdCDEF, and tcbCDEF. The
clcABD genes are responsible for degradation of
3-chlorocatechol and were cloned from plasmid pAC27 of
Pseudomonas putida, which is a 3-chlorobenzoate
(3-CB)-degrading bacterium (9, 11, 22). The
tfdCDEF genes are present on plasmid pJP4 and are
responsible for the degradation of 3,5-dichlorocatechol, which is
produced from 2,4-dichlorophenoxyacetate by the products of the
tfdAB genes in Alcaligenes eutrophus
(Ralstonia eutropha) JMP134 (17, 18, 46). The
tcbCDEF genes are located on plasmid pP51 and are
responsible for the degradation of 3,4,6-trichlorocatechol, generated
from 1,2,4-trichlorobenzene by the products of the
tcbAB genes in Pseudomonas sp. strain P51
(62, 64). These three gene clusters of gram-negative bacteria have apparently evolved from common ancestral chlorocatechol genes (62). Recently, the study of both the catechol and the chlorocatechol ortho-cleavage pathways of Rhodococcus
opacus 1CP has shown that the chlorocatechol
ortho-cleavage genes of this strain have evolved,
independently of those of gram-negative bacteria, from the common
origin of the catechol ortho-cleavage genes in all bacteria
(19, 20).
The worldwide distribution of genes for chlorocatechol degradation has
been suggested by the discovery of several isolates from different
places (4, 6, 10-12, 17, 30, 37, 39, 58, 59, 64) and has
recently been demonstrated more systematically by the studies of
Fulthorpe et al. (23, 24, 33). However, the means of
dissemination of the gene clusters is less clear: there have been only
a few examples of identical or highly homologous plasmids which carried
the modified ortho-pathway genes, indicating that they are
transferred by plasmids (4, 10, 17). Although the similar
operon-like structures of the gene clusters of the modified
ortho-cleavage pathway suggest they might have spread as
units on a transposable element, there has been only one documented example of a transposable element that carries the modified
ortho-pathway genes (35).
In this paper, we report the structure of genes for
chlorocatechol-degrading enzymes of A. eutrophus NH9,
which was isolated in Japan. The nucleotide sequences of the
cbnR-ABCD genes carried by a composite transposon
structure are highly homologous to those of the tcbR-CDEF
genes of strain P51, which was isolated in The Netherlands. This is the
first report, to our knowledge, of nearly identical two-gene clusters
of the modified ortho pathway which were disseminated by
insertion sequences (ISs).
Strains and plasmids.
Cosmid pPSA842 and Escherichia
coli B378 (53) were used to construct a genomic library
of plasmid pENH91 of A. eutrophus NH9. Plasmid pKT230
(5) was used for subcloning of the genes for catabolic
enzymes. P. putida KT2440 (5) was used as the host strain for growth tests of complementation by subcloned fragments of DNA. The 3.3-kb SalI-SphI fragment containing
DR2 was inserted into pUC18 to yield pEUDR2. The 4.7-kb
EcoRI fragment containing DR1 was inserted into pBluescript
KS( Media and culture conditions.
The ability of strain NH9 to
grow on dichlorobenzene and on 1,2,4-trichlorobenzene was tested
basically as described by van der Meer et al. (64). A
preculture of NH9 was inoculated into liquid basal salts medium
(45) supplemented with either 3.5 mmol of 1,2- or
1,4-dichlorobenzene/liter or 3.2 mmol of 1,2,4-trichlorobenzene/liter and was incubated at 30°C.
Cloning of genes for catabolism of 3-CB.
Plasmid DNA was
isolated from strain NH9 as described previously (45). It
was partially digested with Sau3A to generate fragments
predominantly of 30 to 50 kb and subjected to centrifugation in a 10 to
40% sucrose density gradient. Fractions containing DNA fragments of 30 to 50 kb were pooled. This DNA was inserted into
BamHI-digested broad-host-range cosmid pPSA842, packaged with a packaging extract (Gigapack Plus; Stratagene) by the procedure described by the manufacturer, and transduced into E. coli
B378. The individual cosmid clones were mobilized into strain NH9D, a
3-CB Manipulation of DNA.
Subcloning and sequencing of DNA
fragments and hybridization were performed as described elsewhere
(51) unless otherwise stated. Conjugation was performed as
described by Franklin (21). Southern hybridization
experiments for cloning the 3.7-kb SalI fragment from strain
P51 were performed with a digoxigenin labeling kit (Boehringer
Mannheim, Mannheim, Germany) according to the protocol from the
manufacturer. Sequences were determined by the dideoxy
chain-termination method (52) with automated sequencers (373A [Perkin-Elmer-Applied Biosystems Inc., Foster City, Calif.], ALFred [Pharmacia, Uppsala, Sweden], and DSQ-1000L [Shimadzu, Kyoto,
Japan]), with the dye-primer or dye-terminator kits supplied by the
respective manufacturers.
Nucleotide sequence accession numbers.
The nucleotide
sequence of the 6,959-bp SacI-KpnI region
containing the cbnR-ABCD genes and the deduced amino acid
sequences have been deposited in the DDBJ, GenBank, and EMBL databases
under accession no. AB019032. The nucleotide sequence containing the
1,300-bp region from strain P51, homologous to part of
IS1600, has been deposited under accession no. AB019033. The
nucleotide sequence of orfL is included in the sequence that
contains IS1600 (DR2) (from the SphI site to
outside of the KpnI site of pENH91 [Fig.
1b]) that was deposited previously
(45) and that has been updated with correction of the
sequence of DR2 (accession no. D64144).
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
The Chlorocatechol-Catabolic Transposon Tn5707 of
Alcaligenes eutrophus NH9, Carrying a Gene Cluster Highly
Homologous to That in the 1,2,4-Trichlorobenzene-Degrading
Bacterium Pseudomonas sp. Strain P51, Confers the
Ability To Grow on 3-Chlorobenzoate
ABSTRACT
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References
INTRODUCTION
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References
MATERIALS AND METHODS
Top
Abstract
Introduction
Materials and methods
Results
Discussion
References
) (Stratagene, La Jolla, Calif.) to yield pELDR1. Other bacterial
strains and phages used in this study, and growth conditions, have been
described elsewhere (45).
derivative of NH9 that had been cured of plasmid
pENH91 (45), as described by Franklin (21). The
transconjugants were then screened for growth on plates that contained
0.1% 3-CB and streptomycin (25 µg/ml).
View larger version (20K):
[in a new window]
FIG. 1.
(a) Restriction map of pENH91. BI, BamHI; Bg,
BglII; EI, EcoRI; Ev, EcoRV; K,
KpnI; N, NheI; S, SacI. (b) Schematic
representation of regions containing degradative genes and insertion
sequences on plasmids pENH91 and pP51. The open arrows for the
cbn genes, orfL, and the tcb genes
show the locations and the directions of transcription of the ORFs. The
orientation of the open arrows of IS1600, IS1066,
and IS1067 are in agreement with the direction of
transcription of the ORFs within the ISs. The strategies for subcloning
and sequencing the catabolic region on plasmid pENH91 are shown above
the linear map. Fragments shared by cosmid clones or subcloned to
examine the 3-CB phenotype are shown by thin solid lines at the top of
the figure. The thick solid lines above the map of pENH91 indicate DNA
fragments that were sequenced in both directions by using nested sets
of deletions or subcloned restriction fragments. The sequence indicated
by the thick dotted line (a 3.3-kb SalI-SphI
fragment) was reported previously (45) but was corrected in
this study. The thin dotted lines with small arrows indicate subcloned
fragments used to sequence the boundary sites between the sequenced
fragments described above. The small arrows indicate the lengths and
directions of the sequences determined (5' to 3'). Restriction sites
are abbreviated as follows, in addition to those defined in panel a: H,
HindIII; Hc, HincII; Na, NaeI; Nd,
NdeI; P, PstI; Sa, SalI; Sm,
SmaI; and Sp, SphI. The restriction sites in
parentheses are those determined only for subcloning of related
fragments; thus, other sites recognized by such enzymes within the
linear map were not determined before sequencing. The map of pP51 is
based on material in references 62, 63, 65, and
66. Only the SacI and KpnI
sites described in the text are shown for pP51.
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RESULTS |
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Top
Abstract
Introduction
Materials and methods
Results
Discussion
References
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Cloning of genes for catabolic enzymes.
The genes for
degradation of 3-CB by A. eutrophus NH9 are carried on
plasmid pENH91 (45). A library of genes in pENH91 was constructed in E. coli B378 by use of the
broad-host-range cosmid pPSA842. The individual cosmid clones
were mobilized from E. coli into A. eutrophus
NH9D, a 3-CB derivative that had been cured of pENH91
(45), and transconjugants were selected on minimal agar
plates that contained 3-CB and streptomycin. Among about 200 clones
examined, 8 had the 3-CB+ phenotype. A comparison of the
restriction maps of the inserts of the positive clones showed that they
all included a common 13-kb region (Fig. 1b). A physical map of pENH91
was constructed by further restriction analysis (Fig. 1a). For
subcloning, a 9.2-kb SacI fragment (Fig. 1b) from this 13-kb
region was inserted into the broad-host-range vector pKT230 to yield
pEKC1, which was mobilized into A. eutrophus NH9D
(3-CB) and P. putida KT2440. Cells of both
strains harboring pEKC1 grew on 3-CB-supplemented mineral salts agar
plates. A 5.8-kb BamHI-BglII fragment from within
the 9.2-kb SacI fragment did not confer the
3-CB+ phenotype on either strain. These results showed that
the genes for catabolism of 3-CB were located within the 9.2-kb
SacI fragment.
Determination of sequences of genes for degradative enzymes.
Sequencing analysis of the 9.2-kb SacI fragment revealed
seven long open reading frames (ORFs) (Fig. 1b). Six of the ORFs formed
a cluster and exhibited strong homology to ORFs in the following
clusters of chlorocatechol-degradative genes (in order of relatedness):
(i) the tcbR-CDXEF genes on plasmid pP51 in
Pseudomonas sp. strain P51 (62, 63); (ii) the
clcR-ABXDE genes on plasmids pAC27 in P. putida
AC866 and pWR1 in Pseudomonas sp. strain B13 (13, 22,
32); and (iii) the tfdR and tfdCDEF genes
on plasmid pJP4 in A. eutrophus JMP134 (40,
46) ("X" denotes the third ORF in the cluster of degradative
genes tcbCDEF and clcABDE; the functions of
these genes are unknown). In particular, the extent of homology between
the six ORFs of NH9 and ORFs of the tcbR-CDXEF genes of
Pseudomonas sp. strain P51 was very great (Table
1).
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Comparison of the cbn and tcb gene clusters. The regions containing the chlorocatechol-degradative genes of the two plasmids, namely, the 6,959-bp SacI-KpnI regions of pENH91 and pP51, were compared (Fig. 1b and 3 and Table 1). All of the corresponding ORFs were the same length (Table 1). Hence, all the differences between the coding regions of the cbnR-ABCD and tcbR-CDEF genes were substitutions. The cbnA and cbnB genes overlapped by 4 bp. One nucleotide was present between cbnB and the third ORF in the cbn degradative operon. The intergenic region between the third ORF of cbn and cbnC consisted of 21 bp. cbnC overlapped with cbnD by 4 bp. All of these structural features of the cbn genes were the same as those of the corresponding regions of the tcb genes. The nucleotide sequence of the promoter region between cbnR and cbnA (150 bp) was identical to that of the region between tcbR and tcbC.
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Identification of DR1 and DR2 as identical ISs. Two directly oriented homologous elements, DR1 and DR2, were found at the ends of the catabolic gene region on pENH91 (45). We found that the identification in our previous report (45) of one nucleotide, C, at position 850 from the left inverted repeat of DR2 was a sequencing error, and we eliminated it from the sequence of DR2. With this correction, the nucleotide sequences of the two ORFs, ORFA1 and ORFA2 (45), which were homologous to the first and the second half of the IstA gene of IS21 (48), respectively, were found to form one ORF that corresponded to the entire length of the IstA gene. The amino acid sequence deduced from the revised nucleotide sequence showed the highest homology to that of IstA of IS1326 (7) among the ISs of the IS21 family (Fig. 4a). Since DR2 was found to have the perfect structure of an IS of the IS21 group, it was designated IS1600, and the two genes were designated istA and istB (Fig. 4a). Interestingly, although the terminal inverted repeats of IS1326 (26 bp) were considerably longer than those of IS1600 (16 bp), some nucleotides of IS1600 proximal to the inverted repeats matched the nucleotides within the inverted repeats of IS1326 (Fig. 4b).
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An additional ORF in the region between cbnD and DR2. In a 2-kb region between cbnD and DR2, we found an ORF of considerable length that exhibited some similarity to known proteins at the amino acid level (Fig. 1b) and designated it orfL. The deduced amino acid sequence of orfL (240 amino acids [aa]) was homologous to those of many bacterial polypeptides that are known to be components of membrane-bound transport systems for amino acids. In particular, LivF of E. coli (1) and BraG of Pseudomonas aeruginosa PAO (27) were 42 and 39% homologous, respectively, to orfL at the amino acid level. orfL seemed to be complete in length in comparison with LivF (237 aa) and BraG (233 aa). Both LivF and BraG are located at the downstream ends (in the direction of transcription) in their respective gene clusters. Because of the location and apparent direction of transcription of orfL in the region bracketed by the two ISs, it appeared that orfL might have been separated from the rest of the genes in the cluster by an excision event involving IS1600 (DR2).
Cloning and sequencing of a DNA fragment from strain P51 with homology to IS1600. We investigated whether the tcbR-CDEF gene cluster was also associated with an IS1600 (or IS1600-like) sequence on plasmid pP51. Total DNA was extracted from cells of strain P51 that had been grown on Luria-Bertani medium (51) and subjected to Southern hybridization experiments. The presence of plasmid pP51 DNA in the total DNA was confirmed with a 3.5-kb BamHI-PstI fragment containing a part of the cbn gene cluster as the probe. We then used the same membrane and a 2.3-kb HindIII-SphI fragment containing a part of IS1600 (DR2) as the probe. A hybridizing band was observed, and this fragment was cloned from the total DNA of strain P51 into pUC19 as a 3.7-kb SalI fragment. In a subsequent hybridization experiment, using the cloned 3.7-kb SalI fragment as the probe, the SalI-digested total DNA from strain P51 (which retained the tcb genes) and from derivative strains of P51 that had been cured spontaneously of the plasmid P51 after successive cultures on Luria-Bertani liquid medium gave the same pattern of signals. Therefore, it appeared that the cloned 3.7-kb SalI fragment resided on the chromosome of strain P51.
Sequence of a ca. 2-kb region of the cloned 3.7-kb SalI fragment revealed that the region homologous to IS1600 extended for 1,300 bp, with nucleotide homology of 81%. The 1,300-bp region was flanked by nonhomologous marginal regions of ca. 0.2 and 0.5 kb on each side. Thus, the homologous region had not been truncated by the cloning procedure with the SalI sites. The homologous 1,300-bp region started with a 15-bp sequence that resembled the left inverted repeat of IS1600 (Fig. 4b). It was followed by a 115-bp intervening region and then by an ORF (designated orfSA; 1,170 bp) which showed the highest homology with a part of IstA of IS1600 (83% at the amino acid level [Fig. 4a and 5]) in the database. In addition to the fact that the 1,300-bp region seemed to lack the 3' portion of istA, it was obviously not followed by istB and an inverted repeat at the other end. Thus, this fragment seemed to be a remnant of an IS.
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DISCUSSION |
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Abstract
Introduction
Materials and methods
Results
Discussion
References
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In this study, we analyzed the region of plasmid pENH91 that contained the chlorocatechol-degradative genes of A. eutrophus NH9. The chlorocatechol-degradative genes of NH9 (cbnR-ABCD) were found to be highly homologous to those of Pseudomonas sp. strain P51 (tcbR-CDEF) (62, 63). The lengths of the corresponding ORFs, the overlaps of ORFs, and the intervening sequences between ORFs were the same in the two gene clusters. Highly conserved nucleotide sequences and the identical overall structures of the two gene clusters indicated that the horizontal transfer and the divergence at the nucleotide level of the two clusters had occurred relatively recently in the evolutionary history of the clusters of genes in the modified ortho pathway (55).
The frequency of nucleotide divergence between the regions that contained cbnB and tcbD was significantly higher than that between the other corresponding regions of the two clusters (Table 1). This point is of particular interest, since the genes for chloromuconate cycloisomerase are generally more conserved than the other three genes in the cluster of modified ortho-pathway genes (62). If selective pressure on the genes caused such divergence, several features should be noted here. Even though the two clusters were located on plasmids, the G+C content of each gene in the two clusters, which ranged from 61 to 66% in either cluster (Table 1), cannot be regarded as being significantly different from the chromosomal G+C content of A. eutrophus (66.3 to 66.8%) (15) or of species of Pseudomonas (e.g., P. putida, 59.6 to 63.4% [38]). Furthermore, the G+C contents of cbnB (63.3%) and tcbD (63.7%) are similar and are in the middle range of those of all the genes in the respective clusters. Therefore, the nucleotide divergence between cbnB and tcbD cannot be attributed to "GC pressure" from the host. Alternatively, one possible explanation for the rapid divergence of cbnB and tcbD is that some pattern of biased codon usage forced the bacteria in which these genes were located to replace nucleotides in an effort to adapt to their genetic backgrounds (for example, the pool of transfer RNAs). However, we found no significant differences in codon usage patterns between cbnB and tcbD or between cbnB (tcbD) and the other genes in the clusters. At present, it is unknown whether any selective pressure forced the rapid nucleotide divergence of cbnB and tcbD.
The fact that numerous nucleotide substitutions were not limited to the cbnB (tcbD) gene but spanned a region of ca. 1.3 kb that contained cbnB (tcbD) (Fig. 3) suggests that this divergence might have been caused by physical conditions in this region of DNA and not by the genetic nature of cbnB (tcbD). The rate of substitutions is high throughout cbnB (tcbD). Substitutions in the flanking genes tended to be localized in regions proximal to cbnB (tcbD): most of the nucleotide substitutions (18 of 21) between orfXs (next to cbnB or tcbD) were located within 138 bp of the 5' portions of orfXs (1,011 bp), and 5 of 14 nucleotide substitutions between cbnA and tcbC were located within 24 bp of the 3' regions of the genes (756 bp). In the other corresponding parts of the two 6,959-bp SacI-KpnI fragments, the rate of mutation, including substitutions, insertions, and deletions, was one in several hundred base pairs, which might reflect the basal rate of spontaneous mutation. Some unidentified local structure of the DNA in the region containing cbnB (tcbD) might have made this region more vulnerable to substitutions during replication. The high homology maintained at the amino acid level might reflect some constraint for the function of chloromuconate cycloisomerases.
There was a difference between the chloroaromatic compounds that the two strains, NH9 and P51, utilized as substrates for growth. Strain P51 grew on either 1,2- or 1,4-dichlorobenzene, and it also grew on 1,2,4-trichlorobenzene (64). Strain NH9 was tested for growth on these compounds, but it failed to grow in liquid medium in the presence of these chlorobenzenes. On the other hand, strain P51 did not grow on 3-CB (64). This discrepancy in growth substrates might be explained by the difference in "upper-pathway" enzymes available to the two strains. Strain NH9 probably synthesizes enzymes that convert 3-CB to 3-chlorocatechol but not the enzymes that convert chlorobenzenes to any compounds that can be further metabolized. Strain P51 has been reported to synthesize the enzymes that convert chlorobenzenes to the corresponding chlorocatechols (64, 66), but it does not seem to synthesize enzymes that convert chlorobenzoates to chlorocatechols. The recruitment of the homologous chlorocatechol-degradative gene clusters by the two strains, which resulted in the difference in chloroaromatics utilizable as growth substrates, illustrates the economy with which bacteria adapt to xenobiotic compounds.
In contrast to reports on well-described catabolic transposons, such as the toluene transposons on TOL plasmids and the catabolic genes mobilized by IS1071 (44) (for reviews, see references 16, 60, and 67), there has been only one documented example of a transposable element that carries genes for the modified ortho pathway (35). The IS ISJP4 copy A and its incomplete copy C captured the genes tfdS-R-DIICIIEIIFIIBIIK to form a composite transposon on plasmid pJP4 in A. eutrophus JMP134 (35, 36). With regard to their characteristics as transposable elements, there are some differences between Tn5707 and the composite transposon formed by ISJP4. IS1600 of Tn5707 belongs to the IS21 family, while ISJP4 belongs to the IS5 group of the IS4 family (35). The ISJP4 transposon was apparently transposed to pJP4 as a composite transposon, as indicated by the presence of target site duplication at both ends (35). By contrast, the absence of such duplication at both ends of Tn5707 suggests that Tn5707 may not transpose as such a composite unit. Instead, this feature suggests mobilization of the chromosome that resulted from plasmid integration and subsequent excision mediated by IS1600 (45, 67). The existence of orfL in Tn5707 suggests that the origin of the region carried by Tn5707 might have been a bacterial chromosome, since genes for amino acid transport systems have been found on them (1, 27).
The catabolic genes carried by the ISJP4 transposon are different from those carried by Tn5707 in the following ways. Although the tfdDIICIIEIIFII genes are most homologous to the tcbCDEF genes, the homology between the corresponding genes ranged from 58 to 70% at the nucleotide level and from 27 to 65% at the amino acid level. The ISJP4 transposon contains duplicated regulatory genes, tfdR and tfdS (34, 71), as well as additional genes, namely, tfdBII, which might encode chlorophenol monooxygenase, and tfdK, which encodes an active transporter of 2,4-dichlorophenoxyacetate (36, 46).
In addition to the differences in inherent characteristics between Tn5707 and the ISJP4 composite transposon, our present results illustrate the role of the IS elements in the recent dissemination of genes in the modified ortho pathway by the strong homology between the two clusters, cbnR-ABCD and tcbR-CDEF. On the phylogenetic tree of IstAs of the IS21 family, IstA of IS1600 formed a distinct cluster together with orfSA from strain P51, IstA of IS1326, and NmoT (Fig. 5). The branching point of the IstA of IS1326 and the other three elements suggests that these four elements diverged relatively recently in the evolution of the members of IS21 family. IS1326 was found in integrons in antibiotic-resistant clinical isolates (7). NmoT is a putative transposase that corresponds to IstAs and was found proximal to nitrilotriacetate-degradative genes in Chelatobacter heintzii (69). The various origins of the four elements indicate the recent wide distribution of the related IS (-like) elements among bacteria, which in turn raises the possibility that these IS(-like) elements might have been involved in recent genetic rearrangements of various kinds.
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ACKNOWLEDGMENTS |
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We are grateful to Jan Roelof van der Meer for providing strain P51. We also thank Toshiko Kajiwara for assistance with the experiments and Sally M. McFall for helpful comments on the manuscript.
This work was supported by the Program for the Promotion of Basic Research Activities for Innovative BioSciences and by the Ministry of Agriculture, Forestry, and Fisheries of Japan.
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FOOTNOTES |
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* Corresponding author. Mailing address: National Institute of Agro-Environmental Sciences, 3-1-1 Kan-nondai, Tsukuba, Ibaraki 305-8604, Japan. Phone: 81-298-38-8256. Fax: 81-298-38-8199. E-mail: naotow{at}niaes.affrc.go.jp.
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Abstract
Introduction
Materials and methods
Results
Discussion
References
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