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Applied and Environmental Microbiology, May 1999, p. 2246-2249, Vol. 65, No. 5
0099-2240/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Removal of Dibenzofuran, Dibenzo-p-Dioxin, and 2-Chlorodibenzo-p-Dioxin from Soils Inoculated with Sphingomonas sp. Strain RW1

Rolf U. Halden, Barbara G. Halden, and Daryl F. Dwyer^*

Department of Civil Engineering, University of Minnesota, Minneapolis, Minnesota 55455

Received 19 January 1999/Accepted 23 February 1999

	ABSTRACT

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Removal of dibenzofuran, dibenzo-p-dioxin, and 2-chlorodibenzo-p-dioxin (2-CDD) (10 ppm each) from soil microcosms to final concentrations in the parts-per-billion range was affected by the addition of Sphingomonas sp. strain RW1. Rates and extents of removal were influenced by the density of RW1 organisms. For 2-CDD, the rate of removal was dependent on the content of soil organic matter (SOM), with half-life values ranging from 5.8 h (0% SOM) to 26.3 h (5.5% SOM).

	TEXT

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Diaryl ether compounds include several chemicals (e.g., 2,3,7,8-tetrachlorodibenzo-p-dioxin) that are recognized as toxic pollutants and that persist in soils, in part, by sorbing onto organic matter (7, 20) and, to a lesser extent, onto mineral surfaces (12). Aerobic bacteria that degrade dibenzo-p-dioxin (DD), dibenzofuran (DF), carboxydiphenyl ether, and some halogenated derivatives of these chemicals have been isolated (9, 11, 16, 17, 26, 32, 33). Researchers have primarily focused their studies on the activities of these bacteria in liquid culture (10, 18, 19). In one case, degradation of DD and DF in soil microcosms to which Sphingomonas sp. strain RW1 was introduced was observed (25). To determine whether these and similar bacteria have potential for use in in situ bioremediation, data concerning the fate and activity of the bacteria in soils are needed in conjunction with data on the bioavailability of target chemicals (14). These factors were addressed in this study by using soil microcosms and Sphingomonas sp. strain RW1, a bacterium that mineralizes DD and DF as growth substrates and transforms certain mono- and dichlorinated analogs of these chemicals as cometabolic reactions (32).

Bacterial growth conditions. Strain RW1 (no. 6014; German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) was tagged with a Tn5 suicide donor system, pNMBH20, that encodes genes for resistance to kanamycin and for catechol-2,3-dioxygenase (24). This enzyme converts catechol to muconic acid semialdehyde, a product having a bright yellow color, and does so at a higher rate than the catechol-2,3-dioxygenase present in strain RW1. Strain RW1 was routinely grown in the dark (200 rpm, 30°C) by using M9 minimal medium (23) supplemented with trace elements (31) and DF (1 g/liter) as the sole substrate. Solid medium contained agar (15 g/liter) with benzoate (5 mM) as the sole substrate plus kanamycin (50 µg/ml).

Soils. A pale brown, sandy Zimmerman soil (B21 horizon, 18 to 38 cm below surface) was obtained from a previously cultivated field in the Cedar Creek Natural History Area, Minn. A dark soil (A horizon) was obtained from Fort Snelling State Park, St. Paul, Minn. Soils were sieved (2-mm mesh) and stored at 4°C. A quartz sand (Jordan) was obtained from the Minnesota Frac Sand Co., Jordan, Minn. (Table 1). The sand was sieved to obtain a fraction with a particle size distribution of 0.15 to 0.6 mm. Cedar Creek soil was blended with Jordan sand and Fort Snelling soil to obtain various contents of organic matter, ranging from 0 to 5.5% total organic carbon (TOC). Mixtures were air dried, and water was added to a level equal to 60% field capacity.

View this table: [in this window] [in a new window]   TABLE 1.   Components of soils and sand

Soil microcosm studies. Microcosms consisted of either serum bottles (100 ml) plus soil (20 g [dry weight] of soil) or vials (4 ml) plus soil (2 g [dry weight] of soil). DD, 2-chlorodibenzo-p-dioxin (2-CDD) (99% purity; Chemservice, West Chester, Pa.), and DF (Sigma, St. Louis, Mo.) were added to individual microcosms from methanolic stocks 24 h prior to inoculation with strain RW1; strain RW1 does not use methanol as substrate. Bacteria were grown to late log phase, collected by centrifugation (5,000 × g, 20 min), washed twice in saline (0.85% NaCl), and added to the microcosms during mixing. The microcosms were covered with Parafilm and incubated (21°C) in the dark. Densities of strain RW1 and concentrations of DD, DF, and 2-CDD were determined periodically by sacrificing individual microcosms. Data are reported as averages of triplicate determinations.

Bacteria were extracted from soil in an extraction solution (1 ml/g [dry weight] of soil [21]) by agitation on a wrist action shaker (30 min). Extracts were serially diluted in saline; aliquots were then spread on solid medium and incubated (30°C, 7 days). Resultant colonies were sprayed with a solution of catechol (100 mM) to identify strain RW1. The detection limit was 10⁴ CFU/g (dry weight) of soil; the efficiency of recovery was 87%.

DD, DF, and 2-CDD were extracted from soil by addition of acetonitrile (2% H₃PO₄, 1 ml/g [dry weight] of soil) and agitation (60 min). Particles were settled out (10 min), and the supernatant was passed through a PTFE membrane filter (0.2 µm). Aliquots were analyzed by high-pressure liquid chromatography with a Waters LC Module I equipped with a reversed-phase Waters Nova-Pak phenyl column (3.9 by 150 mm; particle size, 4 µm) and a Waters 996 photodiode array detector (_{191-320 nm}). An isocratic solvent of acidified, distilled water (0.1% H₃PO₄) and acetonitrile (50:50) was used. Injection was by automatic sampler; volumes ranged from 10 to 200 µl. Peaks and concentrations were identified by comparison to known standards. Recoveries of DF, DD, and 2-CDD ranged from 89 to 100%; detection limits were 50 ppb. As appropriate, concentrations of chemicals were fit to either a pseudo-first-order rate model, dP/dt = kP, or a second-order rate model, dP/dt = k₂BP, where P is the concentration of substrate, B is the concentration of biomass, t is time, k is the pseudo-first-order rate constant, and k₂ is the second-order rate constant.

Survival of RW1. Densities of strain RW1 (4 × 10⁸ CFU/g [dry weight] of soil) decreased exponentially in soils without substrate amendment at a rate (0.156 ± 0.007 day¹) corresponding to a half-life of 4.4 days. In the presence of DF (10 ppm), the decrease in density was less rapid, at a rate (0.093 ± 0.034 day¹) corresponding to a half-life of 7.5 days. The same rate was observed for several initial densities of the bacterium (4 × 10⁵, 4 × 10⁶, and 4 × 10⁷ CFU/g [dry weight] of soil). In contrast, the presence of DD (10 ppm) did not affect the survival of strain RW1; the density of the bacterium decreased at a rate of 0.148 ± 0.004 day¹, which is similar to that observed in soils without substrate amendment. Densities of strain RW1 decreased dramatically in soils containing 2-CDD (10 ppm), at a rate (0.782 ± 0.045 day¹) corresponding to a half-life of 0.9 days (data not shown).

Degradation of diaryl ether compounds. DF was removed from soils containing strain RW1 (Fig. 1a). The extent of removal was dependent on the initial density of the bacteria: a density of 4 × 10⁷ CFU/g (dry weight) of soil resulted in complete removal of DF (<50 ppb) in 7 days. At lower densities, removal of DF was incomplete after 28 days of incubation. DD was also removed by strain RW1 (Fig. 1b); however, relatively higher densities of cells were required. At the highest density tested (10⁹ CFU/g [dry weight] of soil), 90% of DD was degraded within 24 h; after 3 days, the concentration of DD was below the detection limit (50 ppb). In comparison to DD, 2-CDD was somewhat more persistent in soil (Fig. 1c).

View larger version (18K): [in this window] [in a new window]   FIG. 1.   Removal of DF (a) from soils inoculated with RW1 at densities of 4 × 105 (), 4 × 106 (), and 4 × 107 () CFU/g (dry weight) of soil and of DD (b) and 2-CDD (c) from soils inoculated with RW1 at densities of 107 (), 108 (), and 109 () CFU/g (dry weight) of soil. Control microcosms () received substrates but not RW1.

Removal of 2-CDD by strain RW1 was tested further with soils having varying levels of organic matter; concentrations of 2-CDD were monitored over time (data not shown) and used to calculate rates of degradation (Table 2) with the pseudo-first-order rate model. Rates decreased when comparatively more soil organic matter (SOM) was present; half-life values ranged from 5.8 to 26.3 h. The influence of organic matter became more apparent when rates of degradation for 2-CDD were plotted against SOM (Fig. 2a) and TOC (Fig. 2b). Strong correlations that clearly demonstrated the negative impact of increasing amounts of organic matter on removal rates for 2-CDD were observed.

View this table: [in this window] [in a new window]   TABLE 2.   Pseudo-first-order rate coefficients and half-life values for 2-CDD in soils containing RW1 (109 CFU/g [dry weight] of soil) and various amounts of SOM

View larger version (20K): [in this window] [in a new window]   FIG. 2.   Correlations between pseudo-first-order rates of degradation of 2-CDD by RW1 with SOM (a) and TOC (b). Error bars indicate standard errors obtained by curve fitting.

Data in Fig. 2b were used to obtain densities of strain RW1 that would be required to degrade 10, 50, 90, and 99% of 2-CDD in soils containing various concentrations of organic matter (Fig. 3). Removal rates for 2-CDD were estimated with the correlation in Fig. 2b and the estimated rates divided by cell density (10⁹ CFU/g [dry weight] of soil) to obtain specific transformation rates (k₂) per cell. The second-order rate model was then used to calculate cell densities for each percentage of TOC. This exercise indicated that as the TOC content of soil increased, relatively more biomass was required to achieve the same extent of removal of 2-CDD. For example, it was necessary to add approximately three times more biomass to soil containing 4% TOC than to soil containing 0% TOC to effect removal of 90% 2-CDD.

View larger version (16K): [in this window] [in a new window]   FIG. 3.   Calculated densities of RW1 (CFU/g [dry weight] of soil [dws]) that would be required to achieve 2-CDD removal of 10, 50, 90, and 99% within 24 h in soils with TOC ranging from 0 to 4%.

This study demonstrates three points with respect to the potential use of diaryl ether-degrading bacteria for in situ bioremediation. (i) Bacteria with catabolic pathways for diaryl ethers, as represented by strain RW1, can survive and degrade diaryl ether compounds in soil. (ii) Diaryl ethers can exist as bioavailable chemicals in soils, at least under the conditions used in this study. (iii) Soils having similar densities of diaryl ether-degrading microorganisms, but relatively higher levels of TOC, may exhibit relatively lower rates of degradation of these diaryl ether compounds.

It was interesting that the three diaryl ether compounds used in this study exerted different influences on the survival of strain RW1. Although degradation of DF prolonged the persistence of strain RW1, an increase in bacterial density was not observed, possibly due to a lack of essential nutrients. This suggestion was supported by the observation that nutrient supplementation increased the density of a second diaryl ether-degrading bacterium that was added to Cedar Creek soil (15). In contrast, degradation of DD did not prolong the persistence of strain RW1. This may be related to a difference in utilization rates for DF and DD by strain RW1, as was noted in a similar study (25). In pure culture, the turnover rate for DD (280 µmol h¹ g of protein¹) is relatively slower than the turnover rate for DF (562 µmol h¹ g of protein¹) (data from reference 32) and may not be sufficient to prolong survival of the microorganism.

The survival of strain RW1 was adversely affected by the presence of 2-CDD, suggesting that 2-CCD and/or its metabolites were toxic to the bacterium. Dioxins are typically neither toxic nor inhibitory to microorganisms and have no observable effect on soil respiration (5), microbial activity, and diversity (2). However, during transformation of 2-CDD, 4-chlorocatechol is observed as a dead-end product (32). Chlorinated phenolics are used as bactericides (27), and chlorocatechols are inhibitors of key catabolic enzymes (4, 29, 33). Thus, production of 4-chlorocatechol may have accounted for the relatively poor survival of strain RW1. It appears that by producing toxic intermediates, diaryl ether-degrading microorganisms could be negatively impacted during attempts to elicit in situ bioremediation. Judicious engineering of microorganisms for complete mineralization of diaryl ethers, as has been suggested previously (14, 32), may offer a way around this dilemma.

It is apparent that SOM controlled the rate at which 2-CDD was removed from soil by strain RW1. Bioavailability of hydrophobic pollutants can potentially limit degradation of organic chemicals in soils (1, 3, 30) and is typically controlled by physicochemical processes including diffusion, sorption/desorption, and dissolution (6, 22, 28). Relatively nonpolar chemicals such as dioxins primarily partition into SOM rather than mineral surfaces (7, 8, 13, 20). The correlation between increasing TOC and decreasing rates of removal of 2-CDD suggests that the rate of degradation was limited by sorption/desorption processes. Thus, as TOC content increases, the lower bioavailability of 2-CDD requires that the amount of biomass be increased to elicit effective degradation (Fig. 3). For soils with elevated levels of TOC, this means that the relative cost of remediation via bioaugmentation may be higher, due to the necessity of adding relatively more microorganisms.

	ACKNOWLEDGMENTS

We thank R. Wittich for RW1, N. McClure for pNMBH20, and the Research Analytical Laboratory, University of Minnesota, for soil analyses.

This research was supported by the National Science Foundation (BCS-9318788). Financial support for R.U.H. was provided in part by a University of Minnesota Doctoral Dissertation Fellowship.

	FOOTNOTES

^* Corresponding author. Mailing address: Department of Civil Engineering, 122 Civil Engineering Building, University of Minnesota, 500 Pillsbury Dr., S.E., Minneapolis, MN 55455. Phone: (612) 625-8582. Fax: (612) 626-7750. E-mail: dwyer003{at}tc.umn.edu.

Present address: Lawrence Livermore National Laboratory, Environmental Restoration Division, Livermore, CA 94550.

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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Halden, R. U. Articles by Dwyer, D. F. Search for Related Content PubMed PubMed Citation Articles by Halden, R. U. Articles by Dwyer, D. F. GeoRef GeoRef Citation Agricola Articles by Halden, R. U. Articles by Dwyer, D. F.